Pharmacology - Chook Lab – Research output — University of Texas Southwestern Medical Center

2023

A murine model of hnRNPH2-related neurodevelopmental disorder reveals a mechanism for genetic compensation by Hnrnph1

Korff, A., Yang, X., O'Donovan, K., Gonzalez, A., Teubner, B. J. W., Nakamura, H., Messing, J., Yang, F., Carisey, A. F., Wang, Y. D., Patni, T., Sheppard, H., Zakharenko, S. S., Chook, Y. M., Taylor, J. P. & Kim, H. J., Jul 17 2023, In: Journal of Clinical Investigation. 133, 14, e160309.

Research output: Contribution to journal › Article › peer-review

Open Access

2 Scopus citations

A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 linked to neurodevelopmental disorders

Gonzalez, A., Kim, H. J., Freibaum, B. D., Fung, H. Y. J., Brautigam, C. A., Taylor, J. P. & Chook, Y. M., Aug 3 2023, In: Structure. 31, 8, p. 924-934.e4

Research output: Contribution to journal › Article › peer-review

Open Access

1 Scopus citations

Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex

Jiou, J., Shaffer, J. M., Bernades, N. E., Yee Joyce Fung, H., Dias, J. K., Arcy, S. D. & Chook, Y. M., Jul 18 2023, In: Proceedings of the National Academy of Sciences of the United States of America. 120, 29, e2301199120.

Research output: Contribution to journal › Article › peer-review

Open Access

1 Scopus citations

Molecular basis of RanGTP-activated release of Histones H2A-H2B from Importin-9

Shaffer, J. M., Jiou, J., Tripathi, K., Olaluwoye, O. S., Fung, H. Y. J., Chook, Y. M. & D'Arcy, S., Aug 3 2023, In: Structure. 31, 8, p. 903-911.e3

Research output: Contribution to journal › Article › peer-review

2022

Structure of IMPORTIN-4 bound to the H3–H4–ASF1 histone–histone chaperone complex

Bernardes, N. E., Fung, H. Y. J., Li, Y., Chen, Z. & Chook, Y. M., Sep 20 2022, In: Proceedings of the National Academy of Sciences of the United States of America. 119, 38, e2207177119.

Research output: Contribution to journal › Article › peer-review

Open Access

7 Scopus citations

2021

Mechanism of karyopherin-β2 binding and nuclear import of ALS variants FUS(P525L) and FUS(R495X)

Gonzalez, A., Mannen, T., Çağatay, T., Fujiwara, A., Matsumura, H., Niesman, A. B., Brautigam, C. A., Chook, Y. M. & Yoshizawa, T., Dec 2021, In: Scientific reports. 11, 1, 3754.

Research output: Contribution to journal › Article › peer-review

Open Access

25 Scopus citations

Recurrent XPO1 mutations alter pathogenesis of chronic lymphocytic leukemia

Walker, J. S., Hing, Z. A., Harrington, B., Baumhardt, J., Ozer, H. G., Lehman, A., Giacopelli, B., Beaver, L., Williams, K., Skinner, J. N., Cempre, C. B., Sun, Q., Shacham, S., Stromberg, B. R., Summers, M. K., Abruzzo, L. V., Rassenti, L., Kipps, T. J., Parikh, S., Kay, N. E., & 7 othersRogers, K. A., Woyach, J. A., Coppola, V., Chook, Y. M., Oakes, C., Byrd, J. C. & Lapalombella, R., Dec 2021, In: Journal of Hematology and Oncology. 14, 1, 17.

Research output: Contribution to journal › Article › peer-review

Open Access

29 Scopus citations

2020

Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation

Padavannil, A., Brautigam, C. A. & Chook, Y. M., May 10 2020, In: Bio-protocol. 10, 10, e3625.

Research output: Contribution to journal › Article › peer-review

Open Access

2 Scopus citations

Recognition of nuclear export signals by CRM1 carrying the oncogenic E571K mutation

Baumhardt, J. M., Walker, J. S., Lee, Y., Shakya, B., Brautigam, C. A., Lapalombella, R., Grishin, N. & Chook, Y. M., Aug 1 2020, In: Molecular biology of the cell. 31, 17, p. 1879-1891 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

18 Scopus citations

2019

Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone

Padavannil, A., Sarkar, P., Kim, S. J., Cagatay, T., Jiou, J., Brautigam, C. A., Tomchick, D. R., Sali, A., D’Arcy, S. & Chook, Y. M., Mar 2019, In: eLife. 8, e43630.

Research output: Contribution to journal › Article › peer-review

Open Access

37 Scopus citations

Structural prerequisites for CRM1-dependent nuclear export signaling peptides: accessibility, adapting conformation, and the stability at the binding site

Lee, Y., Pei, J., Baumhardt, J. M., Chook, Y. M. & Grishin, N. V., Dec 1 2019, In: Scientific reports. 9, 1, 6627.

Research output: Contribution to journal › Article › peer-review

Open Access

15 Scopus citations

2018

Active nuclear import and passive nuclear export are the primary determinants of TDP-43 localization

Pinarbasi, E. S., Caǧatay, T., Fung, H. Y. J., Li, Y. C., Chook, Y. M. & Thomas, P. J., Dec 1 2018, In: Scientific reports. 8, 1, 7083.

Research output: Contribution to journal › Article › peer-review

Open Access

83 Scopus citations

Correlation of CRM1-NES affinity with nuclear export activity

Fu, S. C., Fung, H. Y. J., Caǧatay, T., Baumhardt, J. & Chook, Y. M., Aug 15 2018, In: Molecular biology of the cell. 29, 17, p. 2037-2044 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

29 Scopus citations

Günter Blobel 1936-2018

Chook, Y. M., Fontoura, B. M. A. & Rout, M. P., Apr 1 2018, In: Nature structural & molecular biology. 25, 4, p. 297-298 2 p.

Research output: Contribution to journal › Article › peer-review

Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites

Yoshizawa, T., Ali, R., Jiou, J., Fung, H. Y. J., Burke, K. A., Kim, S. J., Lin, Y., Peeples, W. B., Saltzberg, D., Soniat, M., Baumhardt, J. M., Oldenbourg, R., Sali, A., Fawzi, N. L., Rosen, M. K. & Chook, Y. M., Apr 19 2018, In: Cell. 173, 3, p. 693-705.e22

Research output: Contribution to journal › Article › peer-review

Open Access

219 Scopus citations

Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains

Guo, L., Kim, H. J., Wang, H., Monaghan, J., Freyermuth, F., Sung, J. C., O'Donovan, K., Fare, C. M., Diaz, Z., Singh, N., Zhang, Z. C., Coughlin, M., Sweeny, E. A., DeSantis, M. E., Jackrel, M. E., Rodell, C. B., Burdick, J. A., King, O. D., Gitler, A. D., Lagier-Tourenne, C., & 4 othersPandey, U. B., Chook, Y. M., Taylor, J. P. & Shorter, J., Apr 19 2018, In: Cell. 173, 3, p. 677-692.e20

Research output: Contribution to journal › Article › peer-review

Open Access

319 Scopus citations

Structural–functional interactions of NS1-BP protein with the splicing and mRNA export machineries for viral and host gene expression

Zhang, K., Shang, G., Padavannil, A., Wang, J., Sakthivel, R., Chen, X., Kim, M., Thompson, M. G., García-Sastre, A., Lynch, K. W., Chen, Z., Chook, Y. M. & Fontoura, B. M., Dec 26 2018, In: Proceedings of the National Academy of Sciences of the United States of America. 115, 52, p. E12218-E12227

Research output: Contribution to journal › Article › peer-review

Open Access

20 Scopus citations

2017

Nuclear export receptor CRM1 recognizes diverse conformations in nuclear export signals

Fung, H. Y. J., Fu, S. C. & Chook, Y. M., Mar 10 2017, In: eLife. 6, e23961.

Research output: Contribution to journal › Article › peer-review

Open Access

56 Scopus citations

2016

Importin 8 mediates m⁷G cap-sensitive nuclear import of the eukaryotic translation initiation factor eIF4E

Volpon, L., Culjkovic-Kraljacic, B., Osborne, M. J., Ramteke, A., Sun, Q., Niesman, A., Chook, Y. M. & Borden, K. L. B., May 10 2016, In: Proceedings of the National Academy of Sciences of the United States of America. 113, 19, p. 5263-5268 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

43 Scopus citations

Karyopherin-β2 Recognition of a PY-NLS Variant that Lacks the Proline-Tyrosine Motif

Soniat, M. & Chook, Y. M., Oct 4 2016, In: Structure. 24, 10, p. 1802-1809 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

30 Scopus citations

Next-generation XPO1 inhibitor shows improved efficacy and in vivo tolerability in hematological malignancies

Hing, Z. A., Fung, H. Y. J., Ranganathan, P., Mitchell, S., El-Gamal, D., Woyach, J. A., Williams, K., Goettl, V. M., Smith, J., Yu, X., Meng, X., Sun, Q., Cagatay, T., Lehman, A. M., Lucas, D. M., Baloglu, E., Shacham, S., Kauffman, M. G., Byrd, J. C., Chook, Y. M., & 2 othersGarzon, R. & Lapalombella, R., Dec 1 2016, In: Leukemia. 30, 12, p. 2364-2372 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

103 Scopus citations

Recognition elements in the histone H3 and H4 tails for seven different importins

Soniat, M., Caǧatay, T. & Chook, Y. M., Sep 30 2016, In: Journal of Biological Chemistry. 291, 40, p. 21171-21183 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

31 Scopus citations

2015

LocNES: A computational tool for locating classical NESs in CRM1 cargo proteins

Xu, D., Marquis, K., Pei, J., Fu, S. C., Caʇatay, T., Grishin, N. V. & Chook, Y. M., May 1 2015, In: Bioinformatics. 31, 9, p. 1357-1365 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

102 Scopus citations

Nuclear export inhibitors avert progression in preclinical models of inflammatory demyelination

Haines, J. D., Herbin, O., De La Hera, B., Vidaurre, O. G., Moy, G. A., Sun, Q., Fung, H. Y. J., Albrecht, S., Alexandropoulos, K., McCauley, D., Chook, Y. M., Kuhlmann, T., Kidd, G. J., Shacham, S. & Casaccia, P., Apr 28 2015, In: Nature neuroscience. 18, 4, p. 511-520 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

72 Scopus citations

Structural determinants of nuclear export signal orientation in binding to exportin CRM1

Fung, H. Y. J., Fu, S. C., Brautigam, C. A. & Chook, Y. M., Sep 8 2015, In: eLife. 4, September 2015, e10034.

Research output: Contribution to journal › Article › peer-review

Open Access

61 Scopus citations

2014

Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration

Bernis, C., Swift-Taylor, B., Nord, M., Carmona, S., Chook, Y. M. & Forbes, D. J., Apr 1 2014, In: Molecular biology of the cell. 25, 7, p. 992-1009 18 p.

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

2013

Antileukemic activity of nuclear export inhibitors that spare normal hematopoietic cells

Etchin, J., Sun, Q., Kentsis, A., Farmer, A., Zhang, Z. C., Sanda, T., Mansour, M. R., Barcelo, C., McCauley, D., Kauffman, M., Shacham, S., Christie, A. L., Kung, A. L., Rodig, S. J., Chook, Y. M. & Look, A. T., Jan 2013, In: Leukemia. 27, 1, p. 66-74 9 p.

Research output: Contribution to journal › Article › peer-review

158 Scopus citations

Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2

Soniat, M., Sampathkumar, P., Collett, G., Gizzi, A. S., Banu, R. N., Bhosle, R. C., Chamala, S., Chowdhury, S., Fiser, A., Glenn, A. S., Hammonds, J., Hillerich, B., Khafizov, K., Love, J. D., Matikainen, B., Seidel, R. D., Toro, R., Rajesh Kumar, P., Bonanno, J. B., Chook, Y. M., & 1 othersAlmo, S. C., Jun 2013, In: Journal of Structural and Functional Genomics. 14, 2, p. 31-35 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

18 Scopus citations

Nuclear export inhibition through covalent conjugation and hydrolysis of Leptomycin B by CRM1

Sun, Q., Carrasco, Y. P., Hu, Y., Guo, X., Mirzaei, H., MacMillan, J. & Chook, Y. M., Jan 22 2013, In: Proceedings of the National Academy of Sciences of the United States of America. 110, 4, p. 1303-1308 6 p.

Research output: Contribution to journal › Article › peer-review

150 Scopus citations

2012

Selective inhibitors of nuclear export show that CRM1/XPO1 is a target in chronic lymphocytic leukemia

Lapalombella, R., Sun, Q., Williams, K., Tangeman, L., Jha, S., Zhong, Y., Goettl, V., Mahoney, E., Berglund, C., Gupta, S., Farmer, A., Mani, R., Johnson, A. J., Lucas, D., Mo, X., Daelemans, D., Sandanayaka, V., Shechter, S., McCauley, D., Shacham, S., & 3 othersKauffman, M., Chook, Y. M. & Byrd, J. C., Nov 29 2012, In: Blood. 120, 23, p. 4621-4634 14 p.

Research output: Contribution to journal › Article › peer-review

245 Scopus citations

Sequence and structural analyses of nuclear export signals in the NESdb database

Xu, D., Farmer, A., Collett, G., Grishin, N. V. & Chook, Y. M., Sep 15 2012, In: Molecular biology of the cell. 23, 18, p. 3677-3693 17 p.

Research output: Contribution to journal › Article › peer-review

111 Scopus citations

Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS)

Zhang, Z. C. & Chook, Y. M., Jul 24 2012, In: Proceedings of the National Academy of Sciences of the United States of America. 109, 30, p. 12017-12021 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

110 Scopus citations

2011

Evolutionary development of redundant nuclear localization signals in the mRNA export factor NXF1

Zhang, Z. C., Satterly, N., Fontoura, B. M. A. & Chook, Y. M., Dec 1 2011, In: Molecular biology of the cell. 22, 23, p. 4657-4668 12 p.

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Importin-α protein binding to a nuclear localization signal of carbohydrate response element-binding protein (ChREBP)

Ge, Q., Nakagawa, T., Max Wynn, R., Chook, Y. M., Miller, B. C. & Uyeda, K., Aug 12 2011, In: Journal of Biological Chemistry. 286, 32, p. 28119-28127 9 p.

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

2009

Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus

Süel, K. E. & Chook, Y. M., Jun 5 2009, In: Journal of Biological Chemistry. 284, 23, p. 15416-15424 9 p.

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Structural basis for assembly and disassembly of the CRM1 nuclear export complex

Dong, X., Biswas, A. & Chook, Y. M., May 2009, In: Nature Structural and Molecular Biology. 16, 5, p. 558-560 3 p.

Research output: Contribution to journal › Article › peer-review

Open Access

79 Scopus citations

Structural basis for leucine-rich nuclear export signal recognition by CRM1

Dong, X., Biswas, A., Süel, K. E., Jackson, L. K., Martinez, R., Gu, H. & Chook, Y. M., Apr 30 2009, In: Nature. 458, 7242, p. 1136-1141 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

264 Scopus citations

2008

Modular organization and combinatorial energetics of proline-tyrosine nuclear localization signals

Süel, K. E., Gu, H. & Chook, Y. M., Jun 2008, In: PLoS biology. 6, 6, p. 1253-1267 15 p., e137.

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

2007

Conformational Heterogeneity of Karyopherinβ2 Is Segmental

Cansizoglu, A. E. & Chook, Y. M., Nov 13 2007, In: Structure. 15, 11, p. 1431-1441 11 p.

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Structure-based design of a pathway-specific nuclear import inhibitor

Cansizoglu, A. E., Lee, B. J., Zhang, Z. C., Fontoura, B. M. A. & Chook, Y. M., May 2007, In: Nature Structural and Molecular Biology. 14, 5, p. 452-454 3 p.

Research output: Contribution to journal › Article › peer-review

135 Scopus citations

2006

Atomic resolution structures in nuclear transport

Süel, K. E., Cansizoglu, A. E. & Chook, Y. M., Aug 2006, In: Methods. 39, 4, p. 342-355 14 p.

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Rules for Nuclear Localization Sequence Recognition by Karyopherinβ2

Lee, B. J., Cansizoglu, A. E., Süel, K. E., Louis, T. H., Zhang, Z. & Chook, Y. M., Aug 4 2006, In: Cell. 126, 3, p. 543-558 16 p.

Research output: Contribution to journal › Article › peer-review

442 Scopus citations

The nucleoporin Nup96 is required for proper expression of interferon-regulated proteins and functions

Faria, A. M. C., Levay, A., Wang, Y., Kamphorst, A. O., Rosa, M. L. P., Nussenzveig, D. R., Balkan, W., Chook, Y. M., Levy, D. E. & Fontoura, B. M. A., Mar 2006, In: Immunity. 24, 3, p. 295-304 10 p.

Research output: Contribution to journal › Article › peer-review

83 Scopus citations

2004

AMP-activated protein kinase-regulated phosphorylation and acetylation of importin α1: Involvement in the nuclear import of RNA-binding protein HuR

Wang, W., Yang, X., Kawai, T., De Silanes, I. L., Mazan-Mamczarz, K., Chen, P., Yuh, M. C., Quensel, C., Köhler, M. & Gorospe, M., Nov 12 2004, In: Journal of Biological Chemistry. 279, 46, p. 48376-48388 13 p.

Research output: Contribution to journal › Article › peer-review