@article{58a342a48c7a4492812dc8e172a19389,
title = "ZIGIR, a Granule-Specific Zn2+ Indicator, Reveals Human Islet α Cell Heterogeneity",
abstract = "Ghazvini Zadeh et al. develop a fluorescent zinc indictor, ZIGIR, that labels Zn2+-rich secretory granules with high specificity and sensitivity. ZIGIR tracks trafficking and exocytosis of native granules, enables sorting of islet α cells and β cells with high purity, and reveals human α cell heterogeneity and high Zn2+ activity in the human somatostatin granule.",
keywords = "FACS islet cell, TIRF imaging, ZIGIR, Zinc sensor, ZnT8, alpha cell heterogeneity, beta cell, delta cell, dense core granule, zinc imaging",
author = "{Ghazvini Zadeh}, {Ebrahim H.} and Huang, {Zhi Jiang} and Jing Xia and Daliang Li and Davidson, {Howard W.} and Li, {Wen hong}",
note = "Funding Information: We thank Dr. J. Seemann for sharing NPC-1 and GM130 antibodies; ShangKui Xie, Sonia Fuentes, and Xi Li for islet isolation and biochemical assays; Harrison Kidd, Dr. K. Luby-Phelps, and Dr. M. Mettlen for TIRF imaging; IIDP and ADI for providing isolated human islets; and nPOD for offering human pancreatic sections. This work was supported by grant awards to W.L. from the Welch Foundation ( I-1902 ), JDRF ( 1-SRA-2018-675-S-B ), and NIH ( R01GM132610 ). Confocal imaging and FACS were performed at the Live Cell Imaging Core Facility and the Moody Foundation Flow Cytometry Facility of UT Southwestern, respectively. E.H.G.Z. is a recipient of JDRF postdoctoral fellowship ( 3-PDF-2018-583-A-N ). We devote this work in memory of Dr. Roger Y. Tsien. Funding Information: We thank Dr. J. Seemann for sharing NPC-1 and GM130 antibodies; ShangKui Xie, Sonia Fuentes, and Xi Li for islet isolation and biochemical assays; Harrison Kidd, Dr. K. Luby-Phelps, and Dr. M. Mettlen for TIRF imaging; IIDP and ADI for providing isolated human islets; and nPOD for offering human pancreatic sections. This work was supported by grant awards to W.L. from the Welch Foundation (I-1902), JDRF (1-SRA-2018-675-S-B), and NIH (R01GM132610). Confocal imaging and FACS were performed at the Live Cell Imaging Core Facility and the Moody Foundation Flow Cytometry Facility of UT Southwestern, respectively. E.H.G.Z. is a recipient of JDRF postdoctoral fellowship (3-PDF-2018-583-A-N). We devote this work in memory of Dr. Roger Y. Tsien. E.H.G.Z. Z.J.H. J.X. D.L. and W.L. designed and conducted experiments. H.W.D. contributed reagents and offered advice. All authors analyzed and discussed the results. W.L. and E.H.G.Z. wrote the paper. W.L. and E.H.G.Z. are inventors of a patent application concerning ZIGIR. Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = jul,
day = "14",
doi = "10.1016/j.celrep.2020.107904",
language = "English (US)",
volume = "32",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "2",
}