Abstract
Ghazvini Zadeh et al. develop a fluorescent zinc indictor, ZIGIR, that labels Zn2+-rich secretory granules with high specificity and sensitivity. ZIGIR tracks trafficking and exocytosis of native granules, enables sorting of islet α cells and β cells with high purity, and reveals human α cell heterogeneity and high Zn2+ activity in the human somatostatin granule.
Original language | English (US) |
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Article number | 107904 |
Journal | Cell Reports |
Volume | 32 |
Issue number | 2 |
DOIs | |
State | Published - Jul 14 2020 |
Keywords
- FACS islet cell
- TIRF imaging
- ZIGIR
- Zinc sensor
- ZnT8
- alpha cell heterogeneity
- beta cell
- delta cell
- dense core granule
- zinc imaging
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology