@article{e03c1eb4f6b14c49a16b591d0c5ac0a4,
title = "ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase",
abstract = "Williams et al. identify mediators of the amino acid response pathway in leukemia and demonstrate that ZBTB1 is necessary for cellular proliferation specifically under asparagine deprivation. ZBTB1 associates with the promoter of ASNS and promotes the transcriptional upregulation of ASNS under asparagine deprivation. Loss of ZBTB1 sensitizes leukemic cells to L-asparaginase treatment in vitro and in vivo.",
keywords = "ATF4, CRISPR, asparaginase, cancer metabolism, genetic screen, leukemia, transcription",
author = "Williams, {Robert T.} and Rohiverth Guarecuco and Gates, {Leah A.} and Douglas Barrows and Passarelli, {Maria C.} and Bryce Carey and Lou Baudrier and Swarna Jeewajee and Konnor La and Benjamin Prizer and Sohail Malik and Javier Garcia-Bermudez and Zhu, {Xiphias Ge} and Jason Cantor and Henrik Molina and Thomas Carroll and Roeder, {Robert G.} and Omar Abdel-Wahab and Allis, {C. David} and Kıvan{\c c} Birsoy",
note = "Funding Information: We thank all members of the Birsoy lab for helpful suggestions. This research is supported by a grant from the STARR Cancer Consortium . This research was also supported by the Center for Basic and Translational Research on Disorders of the Digestive System through the generosity of the Leona M. and Harry B. Helmsley Charitable Trust . K.B. was supported by DP2 ( DP2 CA228042-01 ), a Chapman-Perelman MMRF grant, and an AACR NextGen grant and is a Pew-Stewart, Searle and Sidney Kimmel Scholar. O.A.-W. has served as a consultant for H3B Biomedicine, Foundation Medicine Inc, Merck, and Janssen and serves on the scientific advisory board of Envisagenics Inc.; O.A.W. has received prior research funding from H3B Biomedicine unrelated to the current manuscript. K.B. serves as a consultant for Nanocare technologies. R.T.W., R.G., and M.C.P. were supported by a Medical Scientist Training Program grant from the National Institute of General Medical Sciences of the National Institutes of Health under award number T32GM007739 to the Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program. M.C.P. was also supported by an F30/31 Predoctoral Fellowship from the National Cancer Institute under award number 1-F30CA247026-01 . R.T.W. was also supported by an F30/F31 Predoctoral Fellowship from the National Cancer Institute under award number 1F30CA247199-01 . J.G.B. is a Special Fellow of the Leukemia & Lymphoma Society. We thank the Rockefeller University Comparative Bioscience Center and the Genomics Resource Center for their support of the animal and genomic studies, respectively. Funding Information: We thank all members of the Birsoy lab for helpful suggestions. This research is supported by a grant from the STARR Cancer Consortium. This research was also supported by the Center for Basic and Translational Research on Disorders of the Digestive System through the generosity of the Leona M. and Harry B. Helmsley Charitable Trust. K.B. was supported by DP2 (DP2 CA228042-01), a Chapman-Perelman MMRF grant, and an AACR NextGen grant and is a Pew-Stewart, Searle and Sidney Kimmel Scholar. O.A.-W. has served as a consultant for H3B Biomedicine, Foundation Medicine Inc, Merck, and Janssen and serves on the scientific advisory board of Envisagenics Inc.; O.A.W. has received prior research funding from H3B Biomedicine unrelated to the current manuscript. K.B. serves as a consultant for Nanocare technologies. R.T.W. R.G. and M.C.P. were supported by a Medical Scientist Training Program grant from the National Institute of General Medical Sciences of the National Institutes of Health under award number T32GM007739 to the Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program. M.C.P. was also supported by an F30/31 Predoctoral Fellowship from the National Cancer Institute under award number 1-F30CA247026-01. R.T.W. was also supported by an F30/F31 Predoctoral Fellowship from the National Cancer Institute under award number 1F30CA247199-01. J.G.B. is a Special Fellow of the Leukemia & Lymphoma Society. We thank the Rockefeller University Comparative Bioscience Center and the Genomics Resource Center for their support of the animal and genomic studies, respectively. Conceptualization, K.B. and R.T.W; Methodology, K.B. R.T.W, S.M. R.R. and D.A.; Formal analysis, K.L. O.A.W. D.B. B.C. L.A.G. and T.C.; Investigation, R.T.W. S.J. X.G.Z. R.G. M.C.P. B.P. and J.G.B.; Writing – Original Draft, K.B. and R.T.W.; Writing – Review and Editing, R.T.W. and K.B.; Funding Acquisition, K.B. and R.T.W.; Resources, L.B. and B.P. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = apr,
day = "7",
doi = "10.1016/j.cmet.2020.03.008",
language = "English (US)",
volume = "31",
pages = "852--861.e6",
journal = "Cell Metabolism",
issn = "1550-4131",
publisher = "Cell Press",
number = "4",
}