TY - JOUR
T1 - ZBP-89, a kruppel-like zinc finger protein, inhibits epidermal growth factor induction of the gastrin promoter
AU - Merchant, Juanita L.
AU - Iyer, Gaitry R.
AU - Taylor, Brigit R.
AU - Kitchen, John R.
AU - Mortensen, Eric R.
AU - Wang, Ziyuan
AU - Flintoft, Rebecca J.
AU - Michel, Jeffrey B.
AU - Bassel-Duby, Rhonda
PY - 1996
Y1 - 1996
N2 - We have shown previously that a GC-rich element (GGGGCGGGGTGGGGGG) conferring epidermal growth factor (EGF) responsiveness to the human gastrin promoter binds Sp1 and additional undefined complexes. A rat GH4 cell line expression library, was screened by using a multimer of the gastrin EGF response element, and three overlapping cDNA clones were identified. The full-length rat cDNA encoded an 89-kDa zinc linger protein (ZBP-89) that was 89% identical to a 49-kDa human factor, htβ, that hinds a GTGGG/CACCC element in T-cell receptor promoters. The conservation of amino acids between the zinc fingers indicates that ZBP-89 is a member of the C2H2 zinc finger family subclass typified by the Drosophila Kruppel protein. ZBP-89 is ubiquitously expressed in normal adult tissues. It binds specifically to the gastrin EGF response element and inhibits EGF induction of the gastrin promoter. Collectively, these results demonstrate that ZBP-89 functions as a repressor of basal and inducible expression of the gastrin gene.
AB - We have shown previously that a GC-rich element (GGGGCGGGGTGGGGGG) conferring epidermal growth factor (EGF) responsiveness to the human gastrin promoter binds Sp1 and additional undefined complexes. A rat GH4 cell line expression library, was screened by using a multimer of the gastrin EGF response element, and three overlapping cDNA clones were identified. The full-length rat cDNA encoded an 89-kDa zinc linger protein (ZBP-89) that was 89% identical to a 49-kDa human factor, htβ, that hinds a GTGGG/CACCC element in T-cell receptor promoters. The conservation of amino acids between the zinc fingers indicates that ZBP-89 is a member of the C2H2 zinc finger family subclass typified by the Drosophila Kruppel protein. ZBP-89 is ubiquitously expressed in normal adult tissues. It binds specifically to the gastrin EGF response element and inhibits EGF induction of the gastrin promoter. Collectively, these results demonstrate that ZBP-89 functions as a repressor of basal and inducible expression of the gastrin gene.
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U2 - 10.1128/MCB.16.12.6644
DO - 10.1128/MCB.16.12.6644
M3 - Article
C2 - 8943318
AN - SCOPUS:0029658599
SN - 0270-7306
VL - 16
SP - 6644
EP - 6653
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 12
ER -