TY - JOUR
T1 - Yeast RNA polymerase II transcription in vitro is inhibited in the presence of nucleotide excision repair
T2 - Complementation of inhibition by Holo-TFIIH and requirement for RAD26
AU - You, Zhaoyang
AU - Feaver, William J.
AU - Friedberg, Errol C.
PY - 1998/5
Y1 - 1998/5
N2 - The Saccharomyces cerevisiae transcription factor IIH (TFIIH) is essential both for transcription by RNA polymerase II (RNAP II) and for nucleotide excision repair (NER) of damaged DNA. We have established cell extracts which support RNAP II transcription from the yeast CYC1 promoter or NER of transcriptionally silent damaged DNA on independent plasmid templates and substrates. When plasmid templates and substrates for both processes are simultaneously incubated with these extracts, transcription is significantly inhibited. This inhibition is strictly dependent on active NER and can be complemented with purified holo-TFIIH. These results suggest that in the presence of active NER, TFIIH is preferentially mobilized from the basal transcription machinery for use in NER. Inhibition of transcription in the presence of active NER requires the RAD26 gene, the yeast homolog of the human Cockayne syndrome group B gene (CSB).
AB - The Saccharomyces cerevisiae transcription factor IIH (TFIIH) is essential both for transcription by RNA polymerase II (RNAP II) and for nucleotide excision repair (NER) of damaged DNA. We have established cell extracts which support RNAP II transcription from the yeast CYC1 promoter or NER of transcriptionally silent damaged DNA on independent plasmid templates and substrates. When plasmid templates and substrates for both processes are simultaneously incubated with these extracts, transcription is significantly inhibited. This inhibition is strictly dependent on active NER and can be complemented with purified holo-TFIIH. These results suggest that in the presence of active NER, TFIIH is preferentially mobilized from the basal transcription machinery for use in NER. Inhibition of transcription in the presence of active NER requires the RAD26 gene, the yeast homolog of the human Cockayne syndrome group B gene (CSB).
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U2 - 10.1128/MCB.18.5.2668
DO - 10.1128/MCB.18.5.2668
M3 - Article
C2 - 9566886
AN - SCOPUS:0031943276
SN - 0270-7306
VL - 18
SP - 2668
EP - 2676
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 5
ER -