@article{1e4c8e5df9664eb2836c917aebf253de,
title = "Xenosiderophore Utilization Promotes Bacteroides thetaiotaomicron Resilience during Colitis",
abstract = "During short-lived perturbations, such as inflammation, the gut microbiota exhibits resilience and reverts to its original configuration. Although microbial access to the micronutrient iron is decreased during colitis, pathogens can scavenge iron by using siderophores. How commensal bacteria acquire iron during gut inflammation is incompletely understood. Curiously, the human commensal Bacteroides thetaiotaomicron does not produce siderophores but grows under iron-limiting conditions using enterobacterial siderophores. Using RNA-seq, we identify B. thetaiotaomicron genes that were upregulated during Salmonella-induced gut inflammation and were predicted to be involved in iron uptake. Mutants in the xusABC locus (BT2063-2065) were defective for xenosiderophore-mediated iron uptake in vitro. In the normal mouse gut, the XusABC system was dispensable, while a xusA mutant colonized poorly during colitis. This work identifies xenosiderophore utilization as a critical mechanism for B. thetaiotaomicron to sustain colonization during inflammation and suggests a mechanism of how interphylum iron metabolism contributes to gut microbiota resilience.",
keywords = "bacteroidetes, gut inflammation, gut microbiota resilience, iron metabolism, siderophore",
author = "Wenhan Zhu and Winter, {Maria G.} and Luisella Spiga and Hughes, {Elizabeth R.} and Rachael Chanin and Aditi Mulgaonkar and Jenelle Pennington and Michelle Maas and Behrendt, {Cassie L.} and Jiwoong Kim and Xiankai Sun and Beiting, {Daniel P.} and Hooper, {Lora V.} and Winter, {Sebastian E.}",
note = "Funding Information: We thank Dr. Eric Hansen (UT Southwestern Medical Center) and Dr. Melissa Ellermann (UT Southwestern Medical Center) for helpful discussions. Work in S.E.W.{\textquoteright}s lab was funded by the NIH ( AI118807 and AI128151 ), the Welch Foundation ( I-1969-20180324 ), the Burroughs Wellcome Fund ( 1017880 ), and a Research Scholar grant ( RSG-17-048-01-MPC ) from the American Cancer Society . Work in L.V.H.{\textquoteright}s lab is supported by the Howard Hughes Medical Institute and by the NIH ( DK070855 ). W.Z. was supported by a Research Fellows Award from the Crohn's and Colitis Foundation ( 454921 ). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the funding agencies. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = mar,
day = "11",
doi = "10.1016/j.chom.2020.01.010",
language = "English (US)",
volume = "27",
pages = "376--388.e8",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "3",
}