Whole Mount In Situ Hybridization in Murine Tissues

Deepika Sharma, Matthew J. Hilton, Courtney M. Karner

Research output: Chapter in Book/Report/Conference proceedingChapter


Whole mount in situ hybridization is a sensitive method used to characterize the spatial and temporal expression of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying gene expression during embryonic development. Here, we describe a procedure for digoxigenin labeled in situ hybridization on whole embryos.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Digoxigenin
  • Gene expression
  • In situ hybridization
  • RNA
  • WISH

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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