@inbook{9db8050a21fc4d1d991895c65f9a5687,
title = "Whole Mount In Situ Hybridization in Murine Tissues",
abstract = "Whole mount in situ hybridization is a sensitive method used to characterize the spatial and temporal expression of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying gene expression during embryonic development. Here, we describe a procedure for digoxigenin labeled in situ hybridization on whole embryos.",
keywords = "Digoxigenin, Gene expression, In situ hybridization, RNA, WISH",
author = "Deepika Sharma and Hilton, {Matthew J.} and Karner, {Courtney M.}",
note = "Funding Information: This work was supported by NIH/NIAMS grants (A032092 and A032121) to M.J.H and (AR071967 and AR076325) to C.M.K. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1028-2_22",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "367--376",
booktitle = "Methods in Molecular Biology",
}