TY - JOUR
T1 - Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine-Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease
AU - Wan, Qian
AU - Peng, Hao
AU - Lyu, Jianxun
AU - Liu, Feng
AU - Cheng, Chuanli
AU - Qiao, Yangzi
AU - Deng, Jie
AU - Zheng, Hairong
AU - Wang, Yi
AU - Zou, Chao
AU - Liu, Xin
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant 81830056, 61901462, 82171904), International partnership program of Chinese Academy of Sciences (Grant 154144KYSB20180063), Guangdong Grant “Key Laboratory for Magnetic Resonance and Multimodality Imaging of Guangdong Province” (2020B1212060051) and Key Technologies for Treatment of Brain Disorders (2018B030332001).
Publisher Copyright:
© 2022 International Society for Magnetic Resonance in Medicine.
PY - 2022/11
Y1 - 2022/11
N2 - Background: Early detection and grading of liver inflammation are important for the management of nonalcoholic fatty liver disease (NAFLD) patients. There is still lack of a noninvasive way for the inflammation characterization in NAFLD. Purpose: To assess liver inflammation grades by water specific T1 (wT1) in a rat model. Study Type: Prospective. Animal model: A total of 65 male rats with methionine-choline-deficient diet-induced NAFLD and 15 male normal rats as control. Field Strength/Sequence: A 3 T; multiecho variable flip angle gradient echo sequence. Assessment: The wT1 and proton density fat fraction were quantified. Inflammation and fibrosis were assessed histologically with H&E and Sirius red stained slices according to the nonalcoholic steatohepatitis scoring system. Inflammation grade was scored with G0/G1/G2/G3 as none/mild/moderate/severe inflammation in NALFD rats. G0 + G1 and G2 + G3 were combined as none-to-mild grade (GL) and moderate-to-severe grade (GH) inflammation groups. Statistical Tests: Analysis of variance (ANOVA), Mann–Whitney U test, Spearman's correlation, and receiver operating characteristic (ROC) analysis were performed. The areas under ROC (AUROC) was used for the diagnostic performance of wT1 in discriminating GH and GL. A P value < 0.01 was considered statistically significant. Results: Seventy-six rats were included in the analysis. The numbers in G0–G3 groups were 5, 16, 13, and 27. wT1 of G0–G3 was 568.55 ± 63.93 msec, 582.53 ± 62.98 msec, 521.21 ± 67.31 msec, and 508.79 ± 60.53 msec. A moderate but significant negative correlation between wT1 and histopathological inflammation grades was observed (rs = −0.42). The wT1 of GH (512.80 ± 62.22 msec) was significantly lower than GL (579.20 ± 61.89 msec). The AUROC of wT1 was 0.79, and the optimal cut-off of wT1 was 562.64 msec (sensitivity: 90%, specificity: 76%), for the discrimination of GL and GH. Data Conclusions: wT1 could differentiate none-to-mild inflammation from moderate-to-severe inflammation in the early stage of the NAFLD rat model. Evidence Level: 1. Technical Efficacy: Stage 1.
AB - Background: Early detection and grading of liver inflammation are important for the management of nonalcoholic fatty liver disease (NAFLD) patients. There is still lack of a noninvasive way for the inflammation characterization in NAFLD. Purpose: To assess liver inflammation grades by water specific T1 (wT1) in a rat model. Study Type: Prospective. Animal model: A total of 65 male rats with methionine-choline-deficient diet-induced NAFLD and 15 male normal rats as control. Field Strength/Sequence: A 3 T; multiecho variable flip angle gradient echo sequence. Assessment: The wT1 and proton density fat fraction were quantified. Inflammation and fibrosis were assessed histologically with H&E and Sirius red stained slices according to the nonalcoholic steatohepatitis scoring system. Inflammation grade was scored with G0/G1/G2/G3 as none/mild/moderate/severe inflammation in NALFD rats. G0 + G1 and G2 + G3 were combined as none-to-mild grade (GL) and moderate-to-severe grade (GH) inflammation groups. Statistical Tests: Analysis of variance (ANOVA), Mann–Whitney U test, Spearman's correlation, and receiver operating characteristic (ROC) analysis were performed. The areas under ROC (AUROC) was used for the diagnostic performance of wT1 in discriminating GH and GL. A P value < 0.01 was considered statistically significant. Results: Seventy-six rats were included in the analysis. The numbers in G0–G3 groups were 5, 16, 13, and 27. wT1 of G0–G3 was 568.55 ± 63.93 msec, 582.53 ± 62.98 msec, 521.21 ± 67.31 msec, and 508.79 ± 60.53 msec. A moderate but significant negative correlation between wT1 and histopathological inflammation grades was observed (rs = −0.42). The wT1 of GH (512.80 ± 62.22 msec) was significantly lower than GL (579.20 ± 61.89 msec). The AUROC of wT1 was 0.79, and the optimal cut-off of wT1 was 562.64 msec (sensitivity: 90%, specificity: 76%), for the discrimination of GL and GH. Data Conclusions: wT1 could differentiate none-to-mild inflammation from moderate-to-severe inflammation in the early stage of the NAFLD rat model. Evidence Level: 1. Technical Efficacy: Stage 1.
KW - liver inflammation
KW - nonalcoholic fatty liver disease
KW - water specific T1 mapping
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U2 - 10.1002/jmri.28131
DO - 10.1002/jmri.28131
M3 - Article
C2 - 35212074
AN - SCOPUS:85125198050
SN - 1053-1807
VL - 56
SP - 1429
EP - 1436
JO - Journal of Magnetic Resonance Imaging
JF - Journal of Magnetic Resonance Imaging
IS - 5
ER -