Abstract
We report on an antibody exclusion method for detecting cell adhesion plaques. Substrata coated by plasma fibronectin (pFN) appeared uniformly fluorescent when analyzed by indirect immunofluorescence using a specific anti-pFN antibody. When cells that had spread on the substrata were permeabilized and analyzed by indirect immunofluorescence, areas of non-fluorescence were apparent beneath the cells and in focal streaks along the cell margins. The nonfluorescent focal streaks were coincident with adhesion plaques visualized by interference reflection microscopy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1031-1036 |
| Number of pages | 6 |
| Journal | Cell Biology International Reports |
| Volume | 4 |
| Issue number | 11 |
| DOIs | |
| State | Published - 1980 |
ASJC Scopus subject areas
- Cell Biology