Vaginal estrogen: A dual-edged sword in postoperative healing of the vaginal wall

Christopher M. Ripperda, Pedro Antonio Maldonado, Jesus F. Acevedo, Patrick W. Keller, Yucel Akgul, John M. Shelton, Ruth Ann Word

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Objective: Reconstructive surgery for pelvic organ prolapse is plagued with high failure rates possibly due to impaired healing or regeneration of the vaginal wall. Here, we tested the hypothesis that postoperative administration of local estrogen, direct injection of mesenchymal stem cells (MSCs), or both lead to improved wound healing of the injured vagina in a menopausal rat model. Methods: Ovariectomized rats underwent surgical injury to the posterior vaginal wall and were randomized to treatment with placebo (n = 41), estrogen cream (n = 47), direct injection of MSCs (n = 39), or both (n = 43). Results: MSCs did not survive after injection and had no appreciable effects on healing of the vaginal wall. Acute postoperative administration of vaginal estrogen altered the response of the vaginal wall to injury with decreased stiffness, decreased collagen content, and decreased expression of transcripts for matrix components in the stromal compartment. Conversely, vaginal estrogen resulted in marked proliferation of the epithelial layer and increased expression of genes related to epithelial barrier function and protease inhibition. Transcripts for genes involved in chronic inflammation and adaptive immunity were also down-regulated in the estrogenized epithelium. Conclusions: Collectively, these data indicate that, in contrast to the reported positive effects of preoperative estrogen on the uninjured vagina, acute administration of postoperative vaginal estrogen has adverse effects on the early phase of healing of the stromal layer. In contrast, postoperative estrogen plays a positive role in healing of the vaginal epithelium after injury.

Original languageEnglish (US)
Pages (from-to)838-849
Number of pages12
Issue number7
StatePublished - Jul 1 2017


  • Estrogen
  • Mesenchymal stem cells
  • Postmenopausal
  • Rat vagina

ASJC Scopus subject areas

  • Obstetrics and Gynecology


Dive into the research topics of 'Vaginal estrogen: A dual-edged sword in postoperative healing of the vaginal wall'. Together they form a unique fingerprint.

Cite this