UV cross-linking of interacting RNA and protein in cultured cells

Emi Sei; Nicholas K. Conrad

doi:10.1016/B978-0-12-420120-0.00004-9

UV cross-linking of interacting RNA and protein in cultured cells

Emi Sei, Nicholas K. Conrad

Research output: Chapter in Book/Report/Conference proceeding › Chapter

11 Scopus citations

Abstract

RNA-protein interactions play indispensable roles in the regulation of cellular functions. Biochemical characterization of these complexes is often done by immunoprecipitation (IP) of RNA-binding proteins (RBPs) followed by identification of co-immunoprecipitated RNAs. This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells.

Original language	English (US)
Title of host publication	Laboratory Methods in Enzymology
Subtitle of host publication	Protein Part B
Publisher	Academic Press Inc.
Pages	53-66
Number of pages	14
ISBN (Print)	9780124201200
DOIs	https://doi.org/10.1016/B978-0-12-420120-0.00004-9
State	Published - 2014

Publication series

Name	Methods in Enzymology
Volume	539
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/B978-0-12-420120-0.00004-9

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title = "UV cross-linking of interacting RNA and protein in cultured cells",

abstract = "RNA-protein interactions play indispensable roles in the regulation of cellular functions. Biochemical characterization of these complexes is often done by immunoprecipitation (IP) of RNA-binding proteins (RBPs) followed by identification of co-immunoprecipitated RNAs. This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells.",

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AB - RNA-protein interactions play indispensable roles in the regulation of cellular functions. Biochemical characterization of these complexes is often done by immunoprecipitation (IP) of RNA-binding proteins (RBPs) followed by identification of co-immunoprecipitated RNAs. This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells.

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ER -

UV cross-linking of interacting RNA and protein in cultured cells

Abstract

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