UV cross-linking of interacting RNA and protein in cultured cells

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations


RNA-protein interactions play indispensable roles in the regulation of cellular functions. Biochemical characterization of these complexes is often done by immunoprecipitation (IP) of RNA-binding proteins (RBPs) followed by identification of co-immunoprecipitated RNAs. This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part B
PublisherAcademic Press Inc.
Number of pages14
ISBN (Print)9780124201200
StatePublished - 2014

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


Dive into the research topics of 'UV cross-linking of interacting RNA and protein in cultured cells'. Together they form a unique fingerprint.

Cite this