Abstract
Affinity-purified rabbit antibody to human lambda and kappa chains (RαHλκ) was conjugated to the A-chain of the plant toxin, ricin. The resulting immunotoxin (RαHλκ-A) killed cells from the tumor cell line Daudi, which bears surface immunoglobulin, but was nontoxic to the CFU-E, BFU-E, and CFU-GM of human bone marrow. RαHλκ-A eliminated 99% of clonogenic Daudi cells that had been mixed with marrow cells in vitro, without demonstrable toxicity to hematopoietic cells. Thus, in vitro treatment of marrow with RαHλκ-A may increase the incidence of cure following autologous bone marrow transplantation for the treatment of human B-cell malignancies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 327-332 |
| Number of pages | 6 |
| Journal | Unknown Journal |
| Volume | 62 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1983 |
ASJC Scopus subject areas
- Biochemistry
- Immunology
- Hematology
- Cell Biology