TY - JOUR
T1 - Use of an antibody-ricin A-chain conjugate to delete neoplastic B cells from human bone marrow
AU - Muirhead, M.
AU - Martin, P. J.
AU - Torok-Storb, B.
AU - Uhr, J. W.
AU - Vitetta, E. S.
PY - 1983
Y1 - 1983
N2 - Affinity-purified rabbit antibody to human lambda and kappa chains (RαHλκ) was conjugated to the A-chain of the plant toxin, ricin. The resulting immunotoxin (RαHλκ-A) killed cells from the tumor cell line Daudi, which bears surface immunoglobulin, but was nontoxic to the CFU-E, BFU-E, and CFU-GM of human bone marrow. RαHλκ-A eliminated 99% of clonogenic Daudi cells that had been mixed with marrow cells in vitro, without demonstrable toxicity to hematopoietic cells. Thus, in vitro treatment of marrow with RαHλκ-A may increase the incidence of cure following autologous bone marrow transplantation for the treatment of human B-cell malignancies.
AB - Affinity-purified rabbit antibody to human lambda and kappa chains (RαHλκ) was conjugated to the A-chain of the plant toxin, ricin. The resulting immunotoxin (RαHλκ-A) killed cells from the tumor cell line Daudi, which bears surface immunoglobulin, but was nontoxic to the CFU-E, BFU-E, and CFU-GM of human bone marrow. RαHλκ-A eliminated 99% of clonogenic Daudi cells that had been mixed with marrow cells in vitro, without demonstrable toxicity to hematopoietic cells. Thus, in vitro treatment of marrow with RαHλκ-A may increase the incidence of cure following autologous bone marrow transplantation for the treatment of human B-cell malignancies.
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U2 - 10.1182/blood.v62.2.327.bloodjournal622327
DO - 10.1182/blood.v62.2.327.bloodjournal622327
M3 - Article
C2 - 6409188
AN - SCOPUS:0020510240
SN - 1744-165X
VL - 62
SP - 327
EP - 332
JO - Seminars in Fetal and Neonatal Medicine
JF - Seminars in Fetal and Neonatal Medicine
IS - 2
ER -