Use of aminopterin in selecting electrically active neuroblastoma cells

J. Peacock, J. Minna, P. Nelson, M. Nirenberg

Research output: Contribution to journalArticlepeer-review

59 Scopus citations


A marked increase in electrical excitability and process formation occurs in the N-18 clone of mouse neuroblastoma as these cells go from the logarithmic phase of growth to the stationary state in confluent cultures. Even more excitable cells can be selected by growth in culture medium containing 10-5 M aminopterin which kills about 90% of the cells. Clone 1A-103 does not develop significant processes or exhibit marked electrical excitability under any of the culture conditions studied. Thus, our results show that one or more of the steps required for generation of the action potential is sensitive to regulation in cultured cells. Methods are presented for obtaining populations of either electrically passive cells or electrically excitable cells which can easily be maintained for several weeks. Clones differ markedly in their capacity to extend processes and their ability to generate action potentials.

Original languageEnglish (US)
Pages (from-to)367-377
Number of pages11
JournalExperimental Cell Research
Issue number2
StatePublished - Aug 1972

ASJC Scopus subject areas

  • Cell Biology


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