Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage

Caixia Guo, Tie Shan Tang, Marzena Bienko, Joanne L. Parker, Aleksandra B. Bielen, Eiichiro Sonoda, Shunichi Takeda, Helle D. Ulrich, Ivan Dikic, Errol C. Friedberg

Research output: Contribution to journalArticlepeer-review

171 Scopus citations


REV1 protein is a eukaryotic member of the Y family of DNA polymerases involved in the tolerance of DNA damage by replicative bypass. The precise role(s) of REV1 in this process is not known. Here we show, by using the yeast two-hybrid assay and the glutathione S-transferase pull-down assay, that mouse REV1 can physically interact with ubiquitin. The association of REV1 with ubiquitin requires the ubiquitin-binding motifs (UBMs) located at the C terminus of REV1. The UBMs also mediate the enhanced association between monoubiquitylated PCNA and REV1. In cells exposed to UV radiation, the association of REV1 with replication foci is dependent on functional UBMs. The UBMs of REV1 are shown to contribute to DNA damage tolerance and damage-induced mutagenesis in vivo.

Original languageEnglish (US)
Pages (from-to)8892-8900
Number of pages9
JournalMolecular and cellular biology
Issue number23
StatePublished - Dec 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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