TY - JOUR
T1 - Twenty-four hour oscillation of cAMP in chick pineal cells
T2 - Role of cAMP in the acute and circadian regulation of melatonin production
AU - Nikaido, Selene S.
AU - Takahashi, Joseph S.
N1 - Funding Information:
We thank D. E. Nelson and Dr. M. E. Pierce for critical comments on the manuscript. This research was supported by NIMH grant R37 MH39592, Searle Scholars award 85-H-107, and NSF WI award DCB-8451642 to J. 5. T. and by NIMH predoctoral NRSA fellowship F31 MH09572 to S. S. N.
PY - 1989/11
Y1 - 1989/11
N2 - Chick pineal cells contain circadian oscillators that regulate a rhythm of melatonin biosynthesis. We explored the role of cAMP in regulating this melatonin rhythm. Chick pineal cells expressed a 24 hr oscillation of cAMP efflux with a waveform similar to that of melatonin. Elevation of cAMP in chick pineal cells stimulated melatonin. These results suggest that an oscillation of cAMP regulates the rhythm of melatonin. We investigated whether cAMP was a component of the circadian oscillator by determining the effects of 8-Br cAMP pulses on the phase of the circadian melatonin rhythm. Six hour pulses of 8-Br CAMP did not cause steady-state phase shifts of the rhythm. The acute regulation of melatonin by cAMP, the 24 hr oscillation of cAMP, and the inability of CAMP to phase-shift the melatonin rhythm strongly suggest that CAMP acts as an output signal of the circadian oscillator.
AB - Chick pineal cells contain circadian oscillators that regulate a rhythm of melatonin biosynthesis. We explored the role of cAMP in regulating this melatonin rhythm. Chick pineal cells expressed a 24 hr oscillation of cAMP efflux with a waveform similar to that of melatonin. Elevation of cAMP in chick pineal cells stimulated melatonin. These results suggest that an oscillation of cAMP regulates the rhythm of melatonin. We investigated whether cAMP was a component of the circadian oscillator by determining the effects of 8-Br cAMP pulses on the phase of the circadian melatonin rhythm. Six hour pulses of 8-Br CAMP did not cause steady-state phase shifts of the rhythm. The acute regulation of melatonin by cAMP, the 24 hr oscillation of cAMP, and the inability of CAMP to phase-shift the melatonin rhythm strongly suggest that CAMP acts as an output signal of the circadian oscillator.
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U2 - 10.1016/0896-6273(89)90271-7
DO - 10.1016/0896-6273(89)90271-7
M3 - Article
C2 - 2484343
AN - SCOPUS:0024756840
SN - 0896-6273
VL - 3
SP - 609
EP - 619
JO - Neuron
JF - Neuron
IS - 5
ER -