TY - JOUR
T1 - Tumor necrosis factor α/cachectin and interleukin 1β initiate meningeal inflammation
AU - Ramilo, Octavio
AU - Sáez-Llorens, Xavier
AU - Mertsola, Jussi
AU - Jafari, Hamid
AU - Olsen, Kurt D.
AU - Hansen, Eric J.
AU - Yoshinaga, Masaru
AU - Ohkawara, Susumu
AU - Nariuchi, Hideo
AU - McCracken, George H.
PY - 1990/8/1
Y1 - 1990/8/1
N2 - Although previous studies using human cytokines in rabbits and rats have provided evidence of the participation of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) in the meningeal inflammatory cascade, the results obtained by several groups of investigators have been discordant or, at times, contradictory. In the present study, homologous cytokines were applied to the rabbit meningitis model. Intracisternal administration of 102-105 IU of purified rabbit TNF-α (RaTNF-α) produced significant cerebrospinal fluid (CSF) inflammation. A similar response was observed after intracisternal inoculation of 5-200 ng of rabbit recombinant IL-1β (rrIL-1β). Preincubation of these two mediators with their specific antibodies resulted in an almost complete suppression of the CSF inflammatory response. In animals with Haemophilus influenzae type b lipooligosaccharide-induced meningitis, intracisternal administration of anti-rrIL-1β, anti-RaTNF-α, or both resulted in a significant modulation of meningeal inflammation. Simultaneous administration of 103 IU of RaTNF-α and 5 ng of rrIL-1β resulted in a synergistic inflammatory response manifested by a more rapid and significantly increased influx of white blood cells into the CSF compared with results after each cytokine given alone. These data provide evidence for a seminal role of TNF-α and IL-1β in the initial events of meningeal inflammation.
AB - Although previous studies using human cytokines in rabbits and rats have provided evidence of the participation of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) in the meningeal inflammatory cascade, the results obtained by several groups of investigators have been discordant or, at times, contradictory. In the present study, homologous cytokines were applied to the rabbit meningitis model. Intracisternal administration of 102-105 IU of purified rabbit TNF-α (RaTNF-α) produced significant cerebrospinal fluid (CSF) inflammation. A similar response was observed after intracisternal inoculation of 5-200 ng of rabbit recombinant IL-1β (rrIL-1β). Preincubation of these two mediators with their specific antibodies resulted in an almost complete suppression of the CSF inflammatory response. In animals with Haemophilus influenzae type b lipooligosaccharide-induced meningitis, intracisternal administration of anti-rrIL-1β, anti-RaTNF-α, or both resulted in a significant modulation of meningeal inflammation. Simultaneous administration of 103 IU of RaTNF-α and 5 ng of rrIL-1β resulted in a synergistic inflammatory response manifested by a more rapid and significantly increased influx of white blood cells into the CSF compared with results after each cytokine given alone. These data provide evidence for a seminal role of TNF-α and IL-1β in the initial events of meningeal inflammation.
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M3 - Article
C2 - 2373990
AN - SCOPUS:0025078410
SN - 0022-1007
VL - 172
SP - 497
EP - 507
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 2
ER -