TY - JOUR
T1 - Transposable Elements Are Co-opted as Oncogenic Regulatory Elements by Lineage-Specific Transcription Factors in Prostate Cancer
AU - Grillo, Giacomo
AU - Keshavarzian, Tina
AU - Linder, Simon
AU - Arlidge, Christopher
AU - Mout, Lisanne
AU - Nand, Ankita
AU - Teng, Mona
AU - Qamra, Aditi
AU - Zhou, Stanley
AU - Kron, Ken J.
AU - Murison, Alex
AU - Hawley, James R.
AU - Fraser, Michael
AU - van der Kwast, Theodorus H.
AU - Raj, Ganesh V.
AU - He, Housheng Hansen
AU - Zwart, Wilbert
AU - Lupien, Mathieu
N1 - Publisher Copyright:
© 2023 The Authors; Published by the American Association for Cancer Research.
PY - 2023/11/1
Y1 - 2023/11/1
N2 - Transposable elements hold regulatory functions that impact cell fate determination by controlling gene expression. However, little is known about the transcriptional machinery engaged at transposable elements in pluripotent and mature versus oncogenic cell states. Through positional analysis over repetitive DNA sequences of H3K27ac chromatin immunoprecipitation sequencing data from 32 normal cell states, we report pluripotent/stem and mature cell state–specific “regulatory transposable elements.” Pluripotent/stem elements are binding sites for pluripotency factors (e.g., NANOG, SOX2, OCT4). Mature cell elements are docking sites for lineage-specific transcription factors, including AR and FOXA1 in prostate epithelium. Expanding the analysis to prostate tumors, we identify a subset of regulatory transposable elements shared with pluripotent/stem cells, including Tigger3a. Using chromatin editing technology, we show how such elements promote prostate cancer growth by regulating AR transcriptional activity. Collectively, our results suggest that oncogenesis arises from lineage-specific transcription factors hijacking pluripotent/stem cell regulatory transposable elements.
AB - Transposable elements hold regulatory functions that impact cell fate determination by controlling gene expression. However, little is known about the transcriptional machinery engaged at transposable elements in pluripotent and mature versus oncogenic cell states. Through positional analysis over repetitive DNA sequences of H3K27ac chromatin immunoprecipitation sequencing data from 32 normal cell states, we report pluripotent/stem and mature cell state–specific “regulatory transposable elements.” Pluripotent/stem elements are binding sites for pluripotency factors (e.g., NANOG, SOX2, OCT4). Mature cell elements are docking sites for lineage-specific transcription factors, including AR and FOXA1 in prostate epithelium. Expanding the analysis to prostate tumors, we identify a subset of regulatory transposable elements shared with pluripotent/stem cells, including Tigger3a. Using chromatin editing technology, we show how such elements promote prostate cancer growth by regulating AR transcriptional activity. Collectively, our results suggest that oncogenesis arises from lineage-specific transcription factors hijacking pluripotent/stem cell regulatory transposable elements.
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U2 - 10.1158/2159-8290.CD-23-0331
DO - 10.1158/2159-8290.CD-23-0331
M3 - Article
C2 - 37694973
AN - SCOPUS:85175740924
SN - 2159-8274
VL - 13
SP - 2470
EP - 2487
JO - Cancer discovery
JF - Cancer discovery
IS - 11
ER -