TY - JOUR
T1 - Transplantation of normal and DMD myoblasts expressing the telomerase gene in SCID mice
AU - Seigneurin-Venin, Sophie
AU - Bernard, Valérie
AU - Moisset, Pierre Alain
AU - Ouellette, Michel M.
AU - Mouly, Vincent
AU - Di Donna, Silvia
AU - Wright, Woodring E.
AU - Tremblay, Jacques P.
N1 - Funding Information:
The authors thank F. Tardif, M. Goulet, and B. Roy for technical assistance. This work was supported by the Association Franc¸aise contre les Myopathies (AFM) and the Muscular Dystrophy Association (MDA) and The National Institute on Aging (Ago 1992).
PY - 2000/6/7
Y1 - 2000/6/7
N2 - The limited proliferative capacity of dystrophic human myoblasts severely limits their ability to be genetically modified and used for myoblast transplantation. The forced expression of the catalytic subunit of telomerase can prevent telomere erosion and can immortalize different cell types. We thus tested the ability of telomerase to immortalize myoblasts and analyzed the effect of telomerase expression on the success of myoblast transplantation. Telomerase expression did not significantly extend the human myoblast life span. The telomerase expressing myoblasts were nonetheless competent to participate in myofiber formation after infection with the retroviral vector. Although the new fibers obtained are less numerous than after the transplantation of normal myoblasts, these results demonstrate that the forced expression of telomerase does not block the ability of normal or dystrophic myoblasts to differentiate in vivo. It will be now necessary to determine the factors that prevent telomerase from extending the life span of human myoblasts before the potential of this intervention can be fully examined. (C) 2000 Academic Press.
AB - The limited proliferative capacity of dystrophic human myoblasts severely limits their ability to be genetically modified and used for myoblast transplantation. The forced expression of the catalytic subunit of telomerase can prevent telomere erosion and can immortalize different cell types. We thus tested the ability of telomerase to immortalize myoblasts and analyzed the effect of telomerase expression on the success of myoblast transplantation. Telomerase expression did not significantly extend the human myoblast life span. The telomerase expressing myoblasts were nonetheless competent to participate in myofiber formation after infection with the retroviral vector. Although the new fibers obtained are less numerous than after the transplantation of normal myoblasts, these results demonstrate that the forced expression of telomerase does not block the ability of normal or dystrophic myoblasts to differentiate in vivo. It will be now necessary to determine the factors that prevent telomerase from extending the life span of human myoblasts before the potential of this intervention can be fully examined. (C) 2000 Academic Press.
KW - Duchenne muscular dystrophy
KW - Myoblast transplantation
KW - SCID mouse
KW - Telomerase gene expression
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U2 - 10.1006/bbrc.2000.2735
DO - 10.1006/bbrc.2000.2735
M3 - Article
C2 - 10833419
AN - SCOPUS:0034616678
SN - 0006-291X
VL - 272
SP - 362
EP - 369
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -