TY - JOUR
T1 - Transcriptome dynamics during human erythroid differentiation and development
AU - Yang, Yadong
AU - Wang, Hai
AU - Chang, Kai Hsin
AU - Qu, Hongzhu
AU - Zhang, Zhaojun
AU - Xiong, Qian
AU - Qi, Heyuan
AU - Cui, Peng
AU - Lin, Qiang
AU - Ruan, Xiuyan
AU - Yang, Yaran
AU - Li, Yajuan
AU - Shu, Chang
AU - Li, Quanzhen
AU - Wakeland, Edward K.
AU - Yan, Jiangwei
AU - Hu, Songnian
AU - Fang, Xiangdong
N1 - Funding Information:
The authors thank Dr. Yuxia Jiao for critically reading the manuscript. This research was supported by the “Strategic Priority Research Program” of the Chinese Academy of Sciences, Stem Cell and Regenerative Medicine Research ( XDA01040405 to X.F.); the National Key Scientific Instrument and Equipment Development Projects of China ( 2011YQ03013404 to X.F.); the National Basic Research Program (973 Program) ( 2006CB910403 to S.H.); the National Natural Science Foundation of China ( 31371300 to Z.Z. and 31100924 to Y.L.); and the National Institute of Health grants of United States ( DK077864 to K-H.C.).
PY - 2013/11
Y1 - 2013/11
N2 - To explore the mechanisms controlling erythroid differentiation and development, we analyzed the genome-wide transcription dynamics occurring during the differentiation of human embryonic stem cells (HESCs) into the erythroid lineage and development of embryonic to adult erythropoiesis using high throughput sequencing technology. HESCs and erythroid cells at three developmental stages: ESER (embryonic), FLER (fetal), and PBER (adult) were analyzed. Our findings revealed that the number of expressed genes decreased during differentiation, whereas the total expression intensity increased. At each of the three transitions (HESCs-ESERs, ESERs-FLERs, and FLERs-PBERs), many differentially expressed genes were observed, which were involved in maintaining pluripotency, early erythroid specification, rapid cell growth, and cell-cell adhesion and interaction. We also discovered dynamic networks and their central nodes in each transition. Our study provides a fundamental basis for further investigation of erythroid differentiation and development, and has implications in using ESERs for transfusion product in clinical settings.
AB - To explore the mechanisms controlling erythroid differentiation and development, we analyzed the genome-wide transcription dynamics occurring during the differentiation of human embryonic stem cells (HESCs) into the erythroid lineage and development of embryonic to adult erythropoiesis using high throughput sequencing technology. HESCs and erythroid cells at three developmental stages: ESER (embryonic), FLER (fetal), and PBER (adult) were analyzed. Our findings revealed that the number of expressed genes decreased during differentiation, whereas the total expression intensity increased. At each of the three transitions (HESCs-ESERs, ESERs-FLERs, and FLERs-PBERs), many differentially expressed genes were observed, which were involved in maintaining pluripotency, early erythroid specification, rapid cell growth, and cell-cell adhesion and interaction. We also discovered dynamic networks and their central nodes in each transition. Our study provides a fundamental basis for further investigation of erythroid differentiation and development, and has implications in using ESERs for transfusion product in clinical settings.
KW - Cell differentiation
KW - Development
KW - Erythropoiesis
KW - Gene regulatory networks
KW - High-throughput RNA sequencing
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U2 - 10.1016/j.ygeno.2013.09.005
DO - 10.1016/j.ygeno.2013.09.005
M3 - Article
C2 - 24121002
AN - SCOPUS:84890121384
SN - 0888-7543
VL - 102
SP - 431
EP - 441
JO - Genomics
JF - Genomics
IS - 5-6
ER -