Transcriptome dynamics during human erythroid differentiation and development

Yadong Yang, Hai Wang, Kai Hsin Chang, Hongzhu Qu, Zhaojun Zhang, Qian Xiong, Heyuan Qi, Peng Cui, Qiang Lin, Xiuyan Ruan, Yaran Yang, Yajuan Li, Chang Shu, Quanzhen Li, Edward K. Wakeland, Jiangwei Yan, Songnian Hu, Xiangdong Fang

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


To explore the mechanisms controlling erythroid differentiation and development, we analyzed the genome-wide transcription dynamics occurring during the differentiation of human embryonic stem cells (HESCs) into the erythroid lineage and development of embryonic to adult erythropoiesis using high throughput sequencing technology. HESCs and erythroid cells at three developmental stages: ESER (embryonic), FLER (fetal), and PBER (adult) were analyzed. Our findings revealed that the number of expressed genes decreased during differentiation, whereas the total expression intensity increased. At each of the three transitions (HESCs-ESERs, ESERs-FLERs, and FLERs-PBERs), many differentially expressed genes were observed, which were involved in maintaining pluripotency, early erythroid specification, rapid cell growth, and cell-cell adhesion and interaction. We also discovered dynamic networks and their central nodes in each transition. Our study provides a fundamental basis for further investigation of erythroid differentiation and development, and has implications in using ESERs for transfusion product in clinical settings.

Original languageEnglish (US)
Pages (from-to)431-441
Number of pages11
Issue number5-6
StatePublished - Nov 2013


  • Cell differentiation
  • Development
  • Erythropoiesis
  • Gene regulatory networks
  • High-throughput RNA sequencing

ASJC Scopus subject areas

  • Genetics


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