The use of cellular reagents as an adjunct to typing for HLA‐DRw10

Two human T cell lines recognizing DRw10 were generated. One line was directly alloreactive, while a second cloned line recognized mumps virus in the context of a DRw10‐like determinant. In panel studies, the lines responded only to DRw10 cells, and not to DR1 (DRw10‐) cells. When compared to 5 anti‐DRw10 sera, the cellular reagents gave virtually identical results. The generation and use of such cellular reagents may be of particular value for the identification of HLA‐D‐region specificites that are rare, poorly defined, or for which serologic typing reagents are limited. 1984 Blackwell Munksgaard