The primary structure of the 70 kDa subunit of bovine soluble guanylate cyclase

Doris Koesling; Joachim Herz; Heinrich Gausepohl; Feraydoon Niroomand; Klaus Dieter Hinsch; Alexander Mülsch; Eycke Böhme; Günter Schultz; Rainer Frank

doi:10.1016/0014-5793(88)80539-8

The primary structure of the 70 kDa subunit of bovine soluble guanylate cyclase

Doris Koesling, Joachim Herz, Heinrich Gausepohl, Feraydoon Niroomand, Klaus Dieter Hinsch, Alexander Mülsch, Eycke Böhme, Günter Schultz, Rainer Frank

Research output: Contribution to journal › Article › peer-review

129 Scopus citations

Abstract

The primary structure of the 70 kDa subunit of soluble bovine guanylate cyclase, which catalyzes the formation of cyclic GMP from GTP, has been determined. The alignment of six different clones out of two bovine libraries yielded a total of 3.1 kb with a coding region of 1857 bases. The open reading frame encodes a protein of 619 amino acids and a molecular mass of 70.5 kDa. Antibodies raised against a synthetic peptide, which corresponded to the C-terminus of the deduced sequence precipitated guanylate cyclase activity from guanylate cyclase-enriched preparations.

Original language	English (US)
Pages (from-to)	29-34
Number of pages	6
Journal	FEBS Letters
Volume	239
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(88)80539-8
State	Published - Oct 24 1988

Keywords

Guanylate cyclase
Peptide antibody
cDNA sequence

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(88)80539-8

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abstract = "The primary structure of the 70 kDa subunit of soluble bovine guanylate cyclase, which catalyzes the formation of cyclic GMP from GTP, has been determined. The alignment of six different clones out of two bovine libraries yielded a total of 3.1 kb with a coding region of 1857 bases. The open reading frame encodes a protein of 619 amino acids and a molecular mass of 70.5 kDa. Antibodies raised against a synthetic peptide, which corresponded to the C-terminus of the deduced sequence precipitated guanylate cyclase activity from guanylate cyclase-enriched preparations.",

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AU - Koesling, Doris

AU - Herz, Joachim

AU - Gausepohl, Heinrich

AU - Niroomand, Feraydoon

AU - Hinsch, Klaus Dieter

AU - Mülsch, Alexander

AU - Böhme, Eycke

AU - Schultz, Günter

AU - Frank, Rainer

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AB - The primary structure of the 70 kDa subunit of soluble bovine guanylate cyclase, which catalyzes the formation of cyclic GMP from GTP, has been determined. The alignment of six different clones out of two bovine libraries yielded a total of 3.1 kb with a coding region of 1857 bases. The open reading frame encodes a protein of 619 amino acids and a molecular mass of 70.5 kDa. Antibodies raised against a synthetic peptide, which corresponded to the C-terminus of the deduced sequence precipitated guanylate cyclase activity from guanylate cyclase-enriched preparations.

KW - Guanylate cyclase

KW - Peptide antibody

KW - cDNA sequence

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The primary structure of the 70 kDa subunit of bovine soluble guanylate cyclase

Abstract

Keywords

ASJC Scopus subject areas

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