TY - JOUR
T1 - The HLA-dependent expression of testis-organizing H-Y antigen by human male cells
AU - Beutler, Bruce
AU - Nagai, Yukifumi
AU - Ohno, Susumu
AU - Klein, George
AU - Shapiro, Ilya M.
N1 - Funding Information:
This work was supported by grants from the NIH.
PY - 1978/3
Y1 - 1978/3
N2 - The proposal that the stable expression of organogenesis-directing plasma membrane antigens, such as testis-organizing H-Y antigen, requires β2-microglobulin-MHC antigen dimers as anchorage sites was tested on Daudi human Burkitt lymphoma cells [46, XY, 15q-, 14q+, β2-m(-), HLA(-)]. The H-Y antigen level of Daudi was only 20% of that of Raji and Ramos, two human male pseudodiploid Burkitt lymphoma lines that were β2-m(+), HLA(+). When Daudi is hybridized with β2-m(+), HLA(+) cell lines, β2-microglobulin, supplied by the latter, is known to restore the expression of Daudi HLA antigens A10 and BW17. Such restoration of HLA antigen expression markedly elevated H-Y antigen levels in those somatic hybrids. Thus the H-Y antigen level of the Daudi × Raji 8A (♂ × ♂) hybrid became equal to that of TetraRaji-the colcemide-induced Raji tetraploid line. Two independently derived Daudi × HeLa D98 (♂ × ♀) hybrids, DAD 1 and DAD 10, demonstrated even higher H-Y antigen levels comparable to that of normal male peripheral blood lymphocytes.
AB - The proposal that the stable expression of organogenesis-directing plasma membrane antigens, such as testis-organizing H-Y antigen, requires β2-microglobulin-MHC antigen dimers as anchorage sites was tested on Daudi human Burkitt lymphoma cells [46, XY, 15q-, 14q+, β2-m(-), HLA(-)]. The H-Y antigen level of Daudi was only 20% of that of Raji and Ramos, two human male pseudodiploid Burkitt lymphoma lines that were β2-m(+), HLA(+). When Daudi is hybridized with β2-m(+), HLA(+) cell lines, β2-microglobulin, supplied by the latter, is known to restore the expression of Daudi HLA antigens A10 and BW17. Such restoration of HLA antigen expression markedly elevated H-Y antigen levels in those somatic hybrids. Thus the H-Y antigen level of the Daudi × Raji 8A (♂ × ♂) hybrid became equal to that of TetraRaji-the colcemide-induced Raji tetraploid line. Two independently derived Daudi × HeLa D98 (♂ × ♀) hybrids, DAD 1 and DAD 10, demonstrated even higher H-Y antigen levels comparable to that of normal male peripheral blood lymphocytes.
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U2 - 10.1016/0092-8674(78)90324-0
DO - 10.1016/0092-8674(78)90324-0
M3 - Article
C2 - 77737
AN - SCOPUS:0017944869
SN - 0092-8674
VL - 13
SP - 509
EP - 513
JO - Cell
JF - Cell
IS - 3
ER -