TY - JOUR
T1 - The Expression of the Kallikrein Gene Family in the Rat Pituitary
T2 - Oestrogen Effects and the Expression of an Additional Family Member in the Neurointermediate Lobe
AU - Clements, J. A.
AU - Matheson, B. A.
AU - MacDonald, R. J.
AU - Funder, J. W.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1989/6
Y1 - 1989/6
N2 - Using a series of oligonucleotide probes (18 to 21 mers) specific for members of the rat kallikrein/tonin gene family (PS, S1, S2, S3, K1, P1), we have shown by Northern blot analysis that the oestrogen‐dependent kallikrein gene expressed in the male and female rat anterior pituitary is true kallikrein (PS). In addition, we have demonstrated that oestrogen treatment may also induce PS gene expression in the male and female rat neurointermediate lobe. None of the other five rat arginyl‐esteropeptidase genes so far described (S1, S2, S3, K1, P1) was found to be expressed in the anterior pituitary or neurointermediate lobe under these conditions. However, the demonstration of an additional hybridization signal in the male neurointermediate lobe using a relatively non‐specific PS gene probe suggests the expression of another, as yet uncharacterized, kallikrein gene family member in this tissue.
AB - Using a series of oligonucleotide probes (18 to 21 mers) specific for members of the rat kallikrein/tonin gene family (PS, S1, S2, S3, K1, P1), we have shown by Northern blot analysis that the oestrogen‐dependent kallikrein gene expressed in the male and female rat anterior pituitary is true kallikrein (PS). In addition, we have demonstrated that oestrogen treatment may also induce PS gene expression in the male and female rat neurointermediate lobe. None of the other five rat arginyl‐esteropeptidase genes so far described (S1, S2, S3, K1, P1) was found to be expressed in the anterior pituitary or neurointermediate lobe under these conditions. However, the demonstration of an additional hybridization signal in the male neurointermediate lobe using a relatively non‐specific PS gene probe suggests the expression of another, as yet uncharacterized, kallikrein gene family member in this tissue.
KW - kallikrein
KW - kallikrein mRNA
KW - oestrogen
KW - pituitary
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U2 - 10.1111/j.1365-2826.1989.tb00103.x
DO - 10.1111/j.1365-2826.1989.tb00103.x
M3 - Article
C2 - 19210455
AN - SCOPUS:0024411901
SN - 0953-8194
VL - 1
SP - 198
EP - 203
JO - Journal of Neuroendocrinology
JF - Journal of Neuroendocrinology
IS - 3
ER -