The effects of cytokines and adherent cells on the interleukin 4-mediated induction of Ia antigens on resting B cells

Kerry Oliver; Peter H. Krammer; Philip W. Tucker; Ellen S. Vitetta

doi:10.1016/0008-8749(87)90185-7

The effects of cytokines and adherent cells on the interleukin 4-mediated induction of Ia antigens on resting B cells

Kerry Oliver, Peter H. Krammer, Philip W. Tucker, Ellen S. Vitetta

Research output: Contribution to journal › Article › peer-review

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Abstract

In this report we have extended our previous studies on interleukin 4 (IL-4) [previously termed B-cell stimulatory factor-1 (BSF-1)]. Our results demonstrate that 8 hr of exposure to IL-4 is sufficient to induce maximal expression of Ia antigens. This increase in expression of Ia antigens on resting B cells is due to the direct action of IL-4 on the B cells since adding or removing adherent cells or utilizing low density cultures of B cells at 50-100/culture had no effect on the IL-4-mediated increase in Ia. Monoclonal anti-IL-4 antibody completely abrogated the Ia-inducing activity of IL-4. A variety of other purified lymphokines including interleukin 2 (IL-2), interleukin 1 (IL-1), and a source of either B-cell differentiation factor for IgM (BCDFμ), or B-cell growth factor II (BCGF II), did not alter the expression of Ia antigens on resting B cells. However, interferon-γ can partially inhibit the IL-4-mediated induction of Ia.

Original language	English (US)
Pages (from-to)	428-436
Number of pages	9
Journal	Cellular Immunology
Volume	106
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(87)90185-7
State	Published - May 1987

ASJC Scopus subject areas

Immunology

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10.1016/0008-8749(87)90185-7

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@article{4dd2333c33e94034bc85a6ff49ab429a,

title = "The effects of cytokines and adherent cells on the interleukin 4-mediated induction of Ia antigens on resting B cells",

abstract = "In this report we have extended our previous studies on interleukin 4 (IL-4) [previously termed B-cell stimulatory factor-1 (BSF-1)]. Our results demonstrate that 8 hr of exposure to IL-4 is sufficient to induce maximal expression of Ia antigens. This increase in expression of Ia antigens on resting B cells is due to the direct action of IL-4 on the B cells since adding or removing adherent cells or utilizing low density cultures of B cells at 50-100/culture had no effect on the IL-4-mediated increase in Ia. Monoclonal anti-IL-4 antibody completely abrogated the Ia-inducing activity of IL-4. A variety of other purified lymphokines including interleukin 2 (IL-2), interleukin 1 (IL-1), and a source of either B-cell differentiation factor for IgM (BCDFμ), or B-cell growth factor II (BCGF II), did not alter the expression of Ia antigens on resting B cells. However, interferon-γ can partially inhibit the IL-4-mediated induction of Ia.",

author = "Kerry Oliver and Krammer, {Peter H.} and Tucker, {Philip W.} and Vitetta, {Ellen S.}",

note = "Funding Information: {\textquoteright} This work is supported by NIH Grants AI-1185 1, AI-2 1229, AI-180 16, NATO Grant 3 13-86, and by the Texas Department of the Ladies Auxiliary, Veterans of Foreign Wars. * To whom reprint requests should be addressed: Department of Microbiology, University of Texas Health Science Center at Dallas, 5323 Harry Hines Blvd., Dallas, TX 75235. {\textquoteright} Abbreviations used: BCDFr(, B-cell diI%erentiation factor for IgM; BCGF II, B-cell growth factor II; BSF-I, B-cell stimulatory factor-l; FACS, fluorescence activated cell sorter; IFN-7, interferon--r; L-4, interleukin 4, H-3, interleukin 3; IG2, interleukin 2; IGl, interleukin 1; MFI, median fluorescence intensity; MV, median volume; PI, propidium iodide; SI, stimulation index.",

year = "1987",

month = may,

doi = "10.1016/0008-8749(87)90185-7",

language = "English (US)",

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journal = "Cellular Immunology",

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T1 - The effects of cytokines and adherent cells on the interleukin 4-mediated induction of Ia antigens on resting B cells

AU - Oliver, Kerry

AU - Krammer, Peter H.

AU - Tucker, Philip W.

AU - Vitetta, Ellen S.

N1 - Funding Information: ’ This work is supported by NIH Grants AI-1185 1, AI-2 1229, AI-180 16, NATO Grant 3 13-86, and by the Texas Department of the Ladies Auxiliary, Veterans of Foreign Wars. * To whom reprint requests should be addressed: Department of Microbiology, University of Texas Health Science Center at Dallas, 5323 Harry Hines Blvd., Dallas, TX 75235. ’ Abbreviations used: BCDFr(, B-cell diI%erentiation factor for IgM; BCGF II, B-cell growth factor II; BSF-I, B-cell stimulatory factor-l; FACS, fluorescence activated cell sorter; IFN-7, interferon--r; L-4, interleukin 4, H-3, interleukin 3; IG2, interleukin 2; IGl, interleukin 1; MFI, median fluorescence intensity; MV, median volume; PI, propidium iodide; SI, stimulation index.

PY - 1987/5

Y1 - 1987/5

N2 - In this report we have extended our previous studies on interleukin 4 (IL-4) [previously termed B-cell stimulatory factor-1 (BSF-1)]. Our results demonstrate that 8 hr of exposure to IL-4 is sufficient to induce maximal expression of Ia antigens. This increase in expression of Ia antigens on resting B cells is due to the direct action of IL-4 on the B cells since adding or removing adherent cells or utilizing low density cultures of B cells at 50-100/culture had no effect on the IL-4-mediated increase in Ia. Monoclonal anti-IL-4 antibody completely abrogated the Ia-inducing activity of IL-4. A variety of other purified lymphokines including interleukin 2 (IL-2), interleukin 1 (IL-1), and a source of either B-cell differentiation factor for IgM (BCDFμ), or B-cell growth factor II (BCGF II), did not alter the expression of Ia antigens on resting B cells. However, interferon-γ can partially inhibit the IL-4-mediated induction of Ia.

AB - In this report we have extended our previous studies on interleukin 4 (IL-4) [previously termed B-cell stimulatory factor-1 (BSF-1)]. Our results demonstrate that 8 hr of exposure to IL-4 is sufficient to induce maximal expression of Ia antigens. This increase in expression of Ia antigens on resting B cells is due to the direct action of IL-4 on the B cells since adding or removing adherent cells or utilizing low density cultures of B cells at 50-100/culture had no effect on the IL-4-mediated increase in Ia. Monoclonal anti-IL-4 antibody completely abrogated the Ia-inducing activity of IL-4. A variety of other purified lymphokines including interleukin 2 (IL-2), interleukin 1 (IL-1), and a source of either B-cell differentiation factor for IgM (BCDFμ), or B-cell growth factor II (BCGF II), did not alter the expression of Ia antigens on resting B cells. However, interferon-γ can partially inhibit the IL-4-mediated induction of Ia.

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The effects of cytokines and adherent cells on the interleukin 4-mediated induction of Ia antigens on resting B cells

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