TY - JOUR
T1 - The developmentally restricted pattern of expression in the male germ line of a murine cyclin A, cyclin A2, suggests roles in both mitotic and meiotic cell cycles
AU - Ravnik, Stuart E.
AU - Wolgemuth, Debra J.
N1 - Funding Information:
sequence data and the kind gift of the cyclin A2 antisera, and Dr. Carrington, Dr. Jan Kitajewski, and Ms. Claire Sweeney for stimulating discussions and critical comments on the manuscript. This work was supported in part by NIH Grants P50 HD 05077 (D.J.W.) and F32 HD 07605 (S.E.R.) and funds from the Lucille Markey Foundation (D.J.W.).
PY - 1996/1/10
Y1 - 1996/1/10
N2 - We have isolated cDNAs encoding a murine cyclin A, designated cyclin A2, and have examined its in vivo expression at the level of both mRNA and protein, with particular focus on the male germ line. Cyclin A2 is expressed in embryos and in a variety of adult tissues, including the testis. In the testis, however, a striking cellular specificity of expression was observed. At both the DNA and protein levels, the predominant sites of cyclin A2 expression were in the germ line stem cells, the spermatogonia, and in highest levels in preleptotene spermatocytes, cells in which premeiotic DNA synthesis occurs. The concurrent localization of cyclin A2 mRNA and protein further suggested that cyclin A2 is regulated at the level of transcription in these cells. The observed cellular specifcity of cyclin A2 expression is consistent with its function during mitosis in the stem cell stage of this lineage, while the restricted meiotic stage localization suggests function in G1/S or S but not in the meiotic divisions per se.
AB - We have isolated cDNAs encoding a murine cyclin A, designated cyclin A2, and have examined its in vivo expression at the level of both mRNA and protein, with particular focus on the male germ line. Cyclin A2 is expressed in embryos and in a variety of adult tissues, including the testis. In the testis, however, a striking cellular specificity of expression was observed. At both the DNA and protein levels, the predominant sites of cyclin A2 expression were in the germ line stem cells, the spermatogonia, and in highest levels in preleptotene spermatocytes, cells in which premeiotic DNA synthesis occurs. The concurrent localization of cyclin A2 mRNA and protein further suggested that cyclin A2 is regulated at the level of transcription in these cells. The observed cellular specifcity of cyclin A2 expression is consistent with its function during mitosis in the stem cell stage of this lineage, while the restricted meiotic stage localization suggests function in G1/S or S but not in the meiotic divisions per se.
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U2 - 10.1006/dbio.1996.0007
DO - 10.1006/dbio.1996.0007
M3 - Article
C2 - 8575639
AN - SCOPUS:0030020371
SN - 0012-1606
VL - 173
SP - 69
EP - 78
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -