TY - JOUR
T1 - The CSC proteins FAP61 and FAP251 build the basal substructures of radial spoke 3 in cilia
AU - Urbanska, Paulina
AU - Song, Kangkang
AU - Joachimiak, Ewa
AU - Krzemien-Ojak, Lucja
AU - Koprowski, Piotr
AU - Hennessey, Todd
AU - Jerka-Dziadosz, Maria
AU - Fabczak, Hanna
AU - Gaertig, Jacek
AU - Nicastro, Daniela
AU - Wloga, Dorota
N1 - Publisher Copyright:
© 2015 Urbanska, Song, et al.
PY - 2015/4/15
Y1 - 2015/4/15
N2 - Dynein motors and regulatory complexes repeat every 96 nm along the length of motile cilia. Each repeat contains three radial spokes, RS1, RS2, and RS3, which transduct signals between the central microtubules and dynein arms. Each radial spoke has a distinct structure, but little is known about the mechanisms of assembly and function of the individual radial spokes. In Chlamydomonas, calmodulin and spoke-associated complex (CSC) is composed of FAP61, FAP91, and FAP251 and has been linked to the base of RS2 and RS3. We show that in Tetrahymena, loss of either FAP61 or FAP251 reduces cell swimming and affects the ciliary waveform and that RS3 is either missing or incomplete, whereas RS1 and RS2 are unaffected. Specifically, FAP251-null cilia lack an arch-like density at the RS3 base, whereas FAP61-null cilia lack an adjacent portion of the RS3 stem region. This suggests that the CSC proteins are crucial for stable and functional assembly of RS3 and that RS3 and the CSC are important for ciliary motility.
AB - Dynein motors and regulatory complexes repeat every 96 nm along the length of motile cilia. Each repeat contains three radial spokes, RS1, RS2, and RS3, which transduct signals between the central microtubules and dynein arms. Each radial spoke has a distinct structure, but little is known about the mechanisms of assembly and function of the individual radial spokes. In Chlamydomonas, calmodulin and spoke-associated complex (CSC) is composed of FAP61, FAP91, and FAP251 and has been linked to the base of RS2 and RS3. We show that in Tetrahymena, loss of either FAP61 or FAP251 reduces cell swimming and affects the ciliary waveform and that RS3 is either missing or incomplete, whereas RS1 and RS2 are unaffected. Specifically, FAP251-null cilia lack an arch-like density at the RS3 base, whereas FAP61-null cilia lack an adjacent portion of the RS3 stem region. This suggests that the CSC proteins are crucial for stable and functional assembly of RS3 and that RS3 and the CSC are important for ciliary motility.
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U2 - 10.1091/mbc.E14-11-1545
DO - 10.1091/mbc.E14-11-1545
M3 - Article
C2 - 25694453
AN - SCOPUS:84928150408
SN - 1059-1524
VL - 26
SP - 1463
EP - 1475
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 8
ER -