The class i myosin MYO1D binds to lipid and protects against colitis

William McAlpine; Kuan Wen Wang; Jin Huk Choi; Miguel San Miguel; Sarah Grace McAlpine; Jamie Russell; Sara Ludwig; Xiaohong Li; Miao Tang; Xiaoming Zhan; Mihwa Choi; Tao Wang; Chun Hui Bu; Anne R. Murray; Eva Marie Y. Moresco; Emre E. Turer; Bruce Beutler

doi:10.1242/dmm.035923

The class i myosin MYO1D binds to lipid and protects against colitis

William McAlpine, Kuan Wen Wang, Jin Huk Choi, Miguel San Miguel, Sarah Grace McAlpine, Jamie Russell, Sara Ludwig, Xiaohong Li, Miao Tang, Xiaoming Zhan, Mihwa Choi, Tao Wang, Chun Hui Bu, Anne R. Murray, Eva Marie Y. Moresco, Emre E. Turer, Bruce Beutler

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Myosin ID (MYO1D) is a member of the class I myosin family. We screened 48,649 third generation (G3) germline mutant mice derived from N-ethyl-N-nitrosourea-mutagenized grandsires for intestinal homeostasis abnormalities after oral administration of dextran sodium sulfate (DSS). We found and validated mutations in Myo1d as a cause of increased susceptibility to DSS-induced colitis. MYO1D is produced in the intestinal epithelium, and the colitis phenotype is dependent on the nonhematopoietic compartment of the mouse. Moreover, MYO1D appears to couple cytoskeletal elements to lipid in an ATP-dependent manner. These findings demonstrate that MYO1D is needed to maintain epithelial integrity and protect against DSSinduced colitis.

Original language	English (US)
Article number	dmm035923
Journal	DMM Disease Models and Mechanisms
Volume	11
Issue number	9
DOIs	https://doi.org/10.1242/dmm.035923
State	Published - Sep 2018

Keywords

Dextran sodium sulfate
Inflammatory bowel disease
N-ethyl-N-nitrosourea

ASJC Scopus subject areas

Neuroscience (miscellaneous)
Medicine (miscellaneous)
Immunology and Microbiology (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)

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10.1242/dmm.035923

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McAlpine, W., Wang, K. W., Choi, J. H., Miguel, M. S., McAlpine, S. G., Russell, J., Ludwig, S., Li, X., Tang, M., Zhan, X., Choi, M., Wang, T., Bu, C. H., Murray, A. R., Moresco, E. M. Y., Turer, E. E., & Beutler, B. (2018). The class i myosin MYO1D binds to lipid and protects against colitis. DMM Disease Models and Mechanisms, 11(9), [dmm035923]. https://doi.org/10.1242/dmm.035923

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title = "The class i myosin MYO1D binds to lipid and protects against colitis",

abstract = "Myosin ID (MYO1D) is a member of the class I myosin family. We screened 48,649 third generation (G3) germline mutant mice derived from N-ethyl-N-nitrosourea-mutagenized grandsires for intestinal homeostasis abnormalities after oral administration of dextran sodium sulfate (DSS). We found and validated mutations in Myo1d as a cause of increased susceptibility to DSS-induced colitis. MYO1D is produced in the intestinal epithelium, and the colitis phenotype is dependent on the nonhematopoietic compartment of the mouse. Moreover, MYO1D appears to couple cytoskeletal elements to lipid in an ATP-dependent manner. These findings demonstrate that MYO1D is needed to maintain epithelial integrity and protect against DSSinduced colitis.",

keywords = "Dextran sodium sulfate, Inflammatory bowel disease, N-ethyl-N-nitrosourea",

author = "William McAlpine and Wang, {Kuan Wen} and Choi, {Jin Huk} and Miguel, {Miguel San} and McAlpine, {Sarah Grace} and Jamie Russell and Sara Ludwig and Xiaohong Li and Miao Tang and Xiaoming Zhan and Mihwa Choi and Tao Wang and Bu, {Chun Hui} and Murray, {Anne R.} and Moresco, {Eva Marie Y.} and Turer, {Emre E.} and Bruce Beutler",

note = "Funding Information: This work was supported by the National Institutes of Health (K08 DK107886 to E.E.T.; AI005184 to W.M.; R37 GM066759 and R01 AI125581 to B.B.), the Crohn{\textquoteright}s and Colitis Foundation of America (2014CCFA00RFA0000090037 to E.E.T) and the Lyda Hill Foundation. Publisher Copyright: {\textcopyright} 2018. Published by The Company of Biologists Ltd | Disease Models & Mechanisms.",

year = "2018",

month = sep,

doi = "10.1242/dmm.035923",

language = "English (US)",

volume = "11",

journal = "DMM Disease Models and Mechanisms",

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AU - McAlpine, William

AU - Wang, Kuan Wen

AU - Choi, Jin Huk

AU - Miguel, Miguel San

AU - McAlpine, Sarah Grace

AU - Russell, Jamie

AU - Ludwig, Sara

AU - Li, Xiaohong

AU - Tang, Miao

AU - Zhan, Xiaoming

AU - Choi, Mihwa

AU - Wang, Tao

AU - Bu, Chun Hui

AU - Murray, Anne R.

AU - Moresco, Eva Marie Y.

AU - Turer, Emre E.

AU - Beutler, Bruce

N1 - Funding Information: This work was supported by the National Institutes of Health (K08 DK107886 to E.E.T.; AI005184 to W.M.; R37 GM066759 and R01 AI125581 to B.B.), the Crohn’s and Colitis Foundation of America (2014CCFA00RFA0000090037 to E.E.T) and the Lyda Hill Foundation. Publisher Copyright: © 2018. Published by The Company of Biologists Ltd | Disease Models & Mechanisms.

PY - 2018/9

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AB - Myosin ID (MYO1D) is a member of the class I myosin family. We screened 48,649 third generation (G3) germline mutant mice derived from N-ethyl-N-nitrosourea-mutagenized grandsires for intestinal homeostasis abnormalities after oral administration of dextran sodium sulfate (DSS). We found and validated mutations in Myo1d as a cause of increased susceptibility to DSS-induced colitis. MYO1D is produced in the intestinal epithelium, and the colitis phenotype is dependent on the nonhematopoietic compartment of the mouse. Moreover, MYO1D appears to couple cytoskeletal elements to lipid in an ATP-dependent manner. These findings demonstrate that MYO1D is needed to maintain epithelial integrity and protect against DSSinduced colitis.

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KW - N-ethyl-N-nitrosourea

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The class i myosin MYO1D binds to lipid and protects against colitis

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