The adenovirus E1A N-terminal repression domain represses transcription from a chromatin template in vitro

Paul M. Loewenstein; Shwu Yuan Wu; Cheng Ming Chiang; Maurice Green

doi:10.1016/j.virol.2012.03.021

The adenovirus E1A N-terminal repression domain represses transcription from a chromatin template in vitro

Paul M. Loewenstein, Shwu Yuan Wu, Cheng Ming Chiang, Maurice Green

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The adenovirus repression domain of E1A 243R at the E1A N-terminus (E1A 1-80) transcriptionally represses genes involved in differentiation and cell cycle progression. E1A 1-80 represses transcription in vitro from naked DNA templates through its interaction with p300 and TFIID. E1A 1-80 can also interact with several chromatin remodeling factors and associates with chromatin in vivo. We show here that E1A 243R and E1A 1-80 can repress transcription from a reconstituted chromatin template in vitro. Temporal analysis reveals strong repression by E1A 1-80 when added at pre-activation, activation and early transcription stages. Interestingly, E1A 1-80 can greatly enhance transcription from chromatin templates, but not from naked DNA, when added at pre-initiation complex (PIC) formation and transcription-initiation stages. These data reveal a new dimension for E1A 1-80's interface with chromatin and may reflect its interaction with key players in PIC formation, p300 and TFIID, and/or possibly a role in chromatin remodeling.

Original language	English (US)
Pages (from-to)	70-75
Number of pages	6
Journal	Virology
Volume	428
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2012.03.021
State	Published - Jun 20 2012

Keywords

Ad E1A
Adenovirus
N-Terminal transcription repression domain
Reconstituted chromatin

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2012.03.021

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title = "The adenovirus E1A N-terminal repression domain represses transcription from a chromatin template in vitro",

abstract = "The adenovirus repression domain of E1A 243R at the E1A N-terminus (E1A 1-80) transcriptionally represses genes involved in differentiation and cell cycle progression. E1A 1-80 represses transcription in vitro from naked DNA templates through its interaction with p300 and TFIID. E1A 1-80 can also interact with several chromatin remodeling factors and associates with chromatin in vivo. We show here that E1A 243R and E1A 1-80 can repress transcription from a reconstituted chromatin template in vitro. Temporal analysis reveals strong repression by E1A 1-80 when added at pre-activation, activation and early transcription stages. Interestingly, E1A 1-80 can greatly enhance transcription from chromatin templates, but not from naked DNA, when added at pre-initiation complex (PIC) formation and transcription-initiation stages. These data reveal a new dimension for E1A 1-80's interface with chromatin and may reflect its interaction with key players in PIC formation, p300 and TFIID, and/or possibly a role in chromatin remodeling.",

keywords = "Ad E1A, Adenovirus, N-Terminal transcription repression domain, Reconstituted chromatin",

author = "Loewenstein, {Paul M.} and Wu, {Shwu Yuan} and Chiang, {Cheng Ming} and Maurice Green",

note = "Funding Information: We thank C.E. Mulhall for insightful editorial assistance. This work was supported by Research Career Award AI-04739 and National Institutes of Health grants CA29561 to M.G. and CA103867 and CA124760 to C.-M.C.",

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doi = "10.1016/j.virol.2012.03.021",

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AU - Loewenstein, Paul M.

AU - Wu, Shwu Yuan

AU - Chiang, Cheng Ming

AU - Green, Maurice

N1 - Funding Information: We thank C.E. Mulhall for insightful editorial assistance. This work was supported by Research Career Award AI-04739 and National Institutes of Health grants CA29561 to M.G. and CA103867 and CA124760 to C.-M.C.

PY - 2012/6/20

Y1 - 2012/6/20

N2 - The adenovirus repression domain of E1A 243R at the E1A N-terminus (E1A 1-80) transcriptionally represses genes involved in differentiation and cell cycle progression. E1A 1-80 represses transcription in vitro from naked DNA templates through its interaction with p300 and TFIID. E1A 1-80 can also interact with several chromatin remodeling factors and associates with chromatin in vivo. We show here that E1A 243R and E1A 1-80 can repress transcription from a reconstituted chromatin template in vitro. Temporal analysis reveals strong repression by E1A 1-80 when added at pre-activation, activation and early transcription stages. Interestingly, E1A 1-80 can greatly enhance transcription from chromatin templates, but not from naked DNA, when added at pre-initiation complex (PIC) formation and transcription-initiation stages. These data reveal a new dimension for E1A 1-80's interface with chromatin and may reflect its interaction with key players in PIC formation, p300 and TFIID, and/or possibly a role in chromatin remodeling.

AB - The adenovirus repression domain of E1A 243R at the E1A N-terminus (E1A 1-80) transcriptionally represses genes involved in differentiation and cell cycle progression. E1A 1-80 represses transcription in vitro from naked DNA templates through its interaction with p300 and TFIID. E1A 1-80 can also interact with several chromatin remodeling factors and associates with chromatin in vivo. We show here that E1A 243R and E1A 1-80 can repress transcription from a reconstituted chromatin template in vitro. Temporal analysis reveals strong repression by E1A 1-80 when added at pre-activation, activation and early transcription stages. Interestingly, E1A 1-80 can greatly enhance transcription from chromatin templates, but not from naked DNA, when added at pre-initiation complex (PIC) formation and transcription-initiation stages. These data reveal a new dimension for E1A 1-80's interface with chromatin and may reflect its interaction with key players in PIC formation, p300 and TFIID, and/or possibly a role in chromatin remodeling.

KW - Ad E1A

KW - Adenovirus

KW - N-Terminal transcription repression domain

KW - Reconstituted chromatin

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The adenovirus E1A N-terminal repression domain represses transcription from a chromatin template in vitro

Abstract

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