TY - JOUR
T1 - Tenascin expression in human glioma cell lines and normal tissues
AU - Ventimiglia, Joseph B.
AU - Wikstrand, Carol J.
AU - Ostrowski, Lawrence E.
AU - Bourdon, Mario A.
AU - Lightner, Virginia A.
AU - Bigner, Darell D.
N1 - Funding Information:
This research was supported by NIH grants CA 11898, CA 32672, NS 20023, 7R29 CA 45586, 1R1 CA 52897, AR 38479, and T32 GM-07171-16. Editorial assistance was rendered by Ann Tama-riz.
PY - 1992/1
Y1 - 1992/1
N2 - Tenascin expression was evaluated in 21 human glioma cell lines and in normal adult tissue extracts by Western and Northern blotting. The cell lines differed in their relative expression of tenascin in the cell-associated and supernatant compartments. Glioma cell line tenascin production was not uniformly stimulated by changes in fetal bovine serum concentration in the growth media. In most glioma cell lines and normal tissue extracts, reducing Western blots and Northern blots revealed two tenascin species, respectively: a major 340 kDa polypeptide and a 9 kb RNA transcript accompanied by a less intense 250 kDa polypeptide and 7 kDa RNA species. In U-87 MG and in normal adult kidney extracts, however, the 250 kDa band and 7 kb transcript were more prominent. Quantitation of tenascin in the glioma lines revealed variable levels that were significantly higher than those in the tissue extracts.
AB - Tenascin expression was evaluated in 21 human glioma cell lines and in normal adult tissue extracts by Western and Northern blotting. The cell lines differed in their relative expression of tenascin in the cell-associated and supernatant compartments. Glioma cell line tenascin production was not uniformly stimulated by changes in fetal bovine serum concentration in the growth media. In most glioma cell lines and normal tissue extracts, reducing Western blots and Northern blots revealed two tenascin species, respectively: a major 340 kDa polypeptide and a 9 kb RNA transcript accompanied by a less intense 250 kDa polypeptide and 7 kDa RNA species. In U-87 MG and in normal adult kidney extracts, however, the 250 kDa band and 7 kb transcript were more prominent. Quantitation of tenascin in the glioma lines revealed variable levels that were significantly higher than those in the tissue extracts.
KW - Alternative mRNA splicing
KW - Extracellular matrix
KW - Glioma
KW - Immunotherapy
KW - Isoforms
KW - Tenascin
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U2 - 10.1016/0165-5728(92)90029-K
DO - 10.1016/0165-5728(92)90029-K
M3 - Article
C2 - 1370958
AN - SCOPUS:0026544079
SN - 0165-5728
VL - 36
SP - 41
EP - 55
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1
ER -