Abstract
Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.
Original language | English (US) |
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Pages (from-to) | 47-54 |
Number of pages | 8 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 735 |
DOIs | |
State | Published - 2011 |
ASJC Scopus subject areas
- Molecular Biology
- Genetics