TY - JOUR
T1 - Techniques for assessing 3-D cell-matrix mechanical interactions in vitro and in vivo
AU - Miron-Mendoza, Miguel
AU - Koppaka, Vindhya
AU - Zhou, Chengxin
AU - Petroll, W. Matthew
N1 - Funding Information:
Supported by NIH R01 EY 013322 and NIH P30 EY020799 , and an unrestricted Grant from Research to Prevent Blindness, Inc., NY, NY .
PY - 2013/10/1
Y1 - 2013/10/1
N2 - Cellular interactions with extracellular matrices (ECM) through the application of mechanical forces mediate numerous biological processes including developmental morphogenesis, wound healing and cancer metastasis. They also play a key role in the cellular repopulation and/or remodeling of engineered tissues and organs. While 2-D studies can provide important insights into many aspects of cellular mechanobiology, cells reside within 3-D ECMs in vivo, and matrix structure and dimensionality have been shown to impact cell morphology, protein organization and mechanical behavior. Global measurements of cell-induced compaction of 3-D collagen matrices can provide important insights into the regulation of overall cell contractility by various cytokines and signaling pathways. However, to understand how the mechanics of cell spreading, migration, contraction and matrix remodeling are regulated at the molecular level, these processes must also be studied in individual cells. Here we review the evolution and application of techniques for imaging and assessing local cell-matrix mechanical interactions in 3-D culture models, tissue explants and living animals.
AB - Cellular interactions with extracellular matrices (ECM) through the application of mechanical forces mediate numerous biological processes including developmental morphogenesis, wound healing and cancer metastasis. They also play a key role in the cellular repopulation and/or remodeling of engineered tissues and organs. While 2-D studies can provide important insights into many aspects of cellular mechanobiology, cells reside within 3-D ECMs in vivo, and matrix structure and dimensionality have been shown to impact cell morphology, protein organization and mechanical behavior. Global measurements of cell-induced compaction of 3-D collagen matrices can provide important insights into the regulation of overall cell contractility by various cytokines and signaling pathways. However, to understand how the mechanics of cell spreading, migration, contraction and matrix remodeling are regulated at the molecular level, these processes must also be studied in individual cells. Here we review the evolution and application of techniques for imaging and assessing local cell-matrix mechanical interactions in 3-D culture models, tissue explants and living animals.
KW - Cell mechanics
KW - Cell migration
KW - Extracellular matrix
KW - Microscopy
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U2 - 10.1016/j.yexcr.2013.06.018
DO - 10.1016/j.yexcr.2013.06.018
M3 - Review article
C2 - 23819988
AN - SCOPUS:84883550861
SN - 0014-4827
VL - 319
SP - 2470
EP - 2480
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 16
ER -