Abstract
Lipid droplets (LDs) are ubiquitous organelles that fulfill essential roles in response to metabolic cues. The identification of several neutral lipid synthesizing and regulatory protein complexes have propelled significant advance on the mechanisms of LD biogenesis in the endo-plasmic reticulum (ER). However, our understanding of signaling networks, especially transcriptional mechanisms, regulating membrane biogenesis is very limited. Here, we show that the nu-trient‐sensing Target of Rapamycin Complex 1 (TORC1) regulates LD formation at a transcriptional level, by targeting DGA1 expression, in a Sit4‐, Mks1‐, and Sfp1‐dependent manner. We show that cytosolic pH (pHc), co‐regulated by the plasma membrane H+‐ATPase Pma1 and the vacuolar ATPase (V‐ATPase), acts as a second messenger, upstream of protein kinase A (PKA), to adjust the localization and activity of the major transcription factor repressor Opi1, which in turn controls the metabolic switch between phospholipid metabolism and lipid storage. Together, this work delineates hitherto unknown molecular mechanisms that couple nutrient availability and pHc to LD formation through a transcriptional circuit regulated by major signaling transduction pathways.
Original language | English (US) |
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Article number | 9017 |
Journal | International journal of molecular sciences |
Volume | 22 |
Issue number | 16 |
DOIs | |
State | Published - Aug 2 2021 |
Externally published | Yes |
Keywords
- Cell signaling
- Lipid droplet
- Membrane biogenesis
- Nutrient
- Transcription
ASJC Scopus subject areas
- Catalysis
- Molecular Biology
- Spectroscopy
- Computer Science Applications
- Physical and Theoretical Chemistry
- Organic Chemistry
- Inorganic Chemistry