Synthesis of biologically active biotinylated muramyl dipeptides

Catherine Leimkuhler Grimes; Daniel K. Podolsky; Erin K. O'Shea

doi:10.1016/j.bmcl.2010.08.056

Synthesis of biologically active biotinylated muramyl dipeptides

Catherine Leimkuhler Grimes, Daniel K. Podolsky, Erin K. O'Shea

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Muramyl dipeptide (MDP) is believed to interact with an innate immune receptor, Nod2. To identify the cellular receptor for MDP, we have synthesized biotinylated MDP isomers and tested the ability of these compounds to activate Nod2 in a cell-based assay. We found that the modification of MDP does not perturb its ability to activate Nod2. These tagged versions of MDP will be useful to identify the cellular receptor of the immunostimulatory molecules.

Original language	English (US)
Pages (from-to)	6061-6063
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	20
Issue number	20
DOIs	https://doi.org/10.1016/j.bmcl.2010.08.056
State	Published - Oct 2010

Keywords

Affinity reagent
Innate immunity
Muramyl dipeptide
Nod2

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2010.08.056

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title = "Synthesis of biologically active biotinylated muramyl dipeptides",

abstract = "Muramyl dipeptide (MDP) is believed to interact with an innate immune receptor, Nod2. To identify the cellular receptor for MDP, we have synthesized biotinylated MDP isomers and tested the ability of these compounds to activate Nod2 in a cell-based assay. We found that the modification of MDP does not perturb its ability to activate Nod2. These tagged versions of MDP will be useful to identify the cellular receptor of the immunostimulatory molecules.",

keywords = "Affinity reagent, Innate immunity, Muramyl dipeptide, Nod2",

author = "Grimes, {Catherine Leimkuhler} and Podolsky, {Daniel K.} and O'Shea, {Erin K.}",

note = "Funding Information: C.L.G. was supported by a postdoctoral fellowship from the Irvington Institute Fellowship Program of the Cancer Research Institute. E.K.O. is an Investigator of the Howard Hughes Medical Institute. D.K.P. was supported by NIH Grant (5R01DK069344). ",

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doi = "10.1016/j.bmcl.2010.08.056",

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T1 - Synthesis of biologically active biotinylated muramyl dipeptides

AU - Grimes, Catherine Leimkuhler

AU - Podolsky, Daniel K.

AU - O'Shea, Erin K.

N1 - Funding Information: C.L.G. was supported by a postdoctoral fellowship from the Irvington Institute Fellowship Program of the Cancer Research Institute. E.K.O. is an Investigator of the Howard Hughes Medical Institute. D.K.P. was supported by NIH Grant (5R01DK069344).

PY - 2010/10

Y1 - 2010/10

N2 - Muramyl dipeptide (MDP) is believed to interact with an innate immune receptor, Nod2. To identify the cellular receptor for MDP, we have synthesized biotinylated MDP isomers and tested the ability of these compounds to activate Nod2 in a cell-based assay. We found that the modification of MDP does not perturb its ability to activate Nod2. These tagged versions of MDP will be useful to identify the cellular receptor of the immunostimulatory molecules.

AB - Muramyl dipeptide (MDP) is believed to interact with an innate immune receptor, Nod2. To identify the cellular receptor for MDP, we have synthesized biotinylated MDP isomers and tested the ability of these compounds to activate Nod2 in a cell-based assay. We found that the modification of MDP does not perturb its ability to activate Nod2. These tagged versions of MDP will be useful to identify the cellular receptor of the immunostimulatory molecules.

KW - Affinity reagent

KW - Innate immunity

KW - Muramyl dipeptide

KW - Nod2

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Synthesis of biologically active biotinylated muramyl dipeptides

Abstract

Keywords

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