@article{ea0244b684ed4c869ec5fdbc5d9b83ef,
title = "Suppression of Ribosomal Pausing by eIF5A Is Necessary to Maintain the Fidelity of Start Codon Selection",
abstract = "Translation initiation codon selection is a highly regulated process that can influence the sequence and activity of a translated protein. Here, Manjunath et al. demonstrate that loss of the translation elongation factor eIF5A triggers widespread translation initiation in 5′ UTRs, uncovering a role for this protein in start codon selection.",
keywords = "5{\textquoteright} untranslated region, MYC, eIF5A, ribosomal pausing, ribosome profiling, translation initiation, upstream translation",
author = "Hema Manjunath and He Zhang and Frederick Rehfeld and Jaeil Han and Chang, {Tsung Cheng} and Mendell, {Joshua T.}",
note = "Funding Information: We thank Rudolph Jaenisch, Didier Trono, and Feng Zhang and for plasmids; Vanessa Schmid, Rachel Bruce, and Caitlin Eaton at the McDermott Center Next Generation Sequencing Core for assistance with high-throughput sequencing; Angie Mobley and the University of Texas (UT) Southwestern (UTSW) Flow Cytometry Core facility for FACS; Frank Gillett for experimental assistance; and Stephen Johnson for software implementation. We also thank Michael Buszczak, Kathryn O'Donnell, and members of the Mendell laboratory for helpful comments on the manuscript. This work was supported by grants from CPRIT (RP160249 to J.T.M. and RP150596 for the UTSW Bioinformatics Core Facility), the NIH (R35CA197311, P30CA142543, and P50CA196516 to J.T.M.), and the Welch Foundation (I-1961-20180324 to J.T.M.). J.T.M. is an investigator of the Howard Hughes Medical Institute. All authors contributed to experimental design and data interpretation. H.M. F.R. J.H. and T.-C.C. performed the molecular biology experiments. H.Z. performed bioinformatics analyses. H.M. and J.T.M. wrote the manuscript. The authors declare no competing interests. Funding Information: We thank Rudolph Jaenisch, Didier Trono, and Feng Zhang and for plasmids; Vanessa Schmid, Rachel Bruce, and Caitlin Eaton at the McDermott Center Next Generation Sequencing Core for assistance with high-throughput sequencing; Angie Mobley and the University of Texas (UT) Southwestern (UTSW) Flow Cytometry Core facility for FACS; Frank Gillett for experimental assistance; and Stephen Johnson for software implementation. We also thank Michael Buszczak, Kathryn O{\textquoteright}Donnell, and members of the Mendell laboratory for helpful comments on the manuscript. This work was supported by grants from CPRIT ( RP160249 to J.T.M. and RP150596 for the UTSW Bioinformatics Core Facility), the NIH ( R35CA197311 , P30CA142543 , and P50CA196516 to J.T.M.), and the Welch Foundation ( I-1961-20180324 to J.T.M.). J.T.M. is an investigator of the Howard Hughes Medical Institute . Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = dec,
day = "3",
doi = "10.1016/j.celrep.2019.10.129",
language = "English (US)",
volume = "29",
pages = "3134--3146.e6",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "10",
}