TY - JOUR
T1 - Supercritical fluid chromatography hyphenated to mass spectrometry for metabolomics applications
AU - Gordillo, Ruth
N1 - Funding Information:
The author thanks Drs. Kevin A. Schug, Christy Gliniak, and Philipp E. Scherer for useful discussions. R. G. is supported by US National Institute of Health (NIH) grant P01 DK088761.
Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2021/1
Y1 - 2021/1
N2 - While supercritical fluid chromatography was developed over 50 years ago, it is only over the past 15 to 20 years that it has become routinely utilized. Along with the commercialization of a new generation of instruments, during the last 20 years supercritical fluid chromatography has improved performance, reliability, and robustness. Supercritical fluid chromatography is fully compatible with mass spectrometric techniques. This review compiles the application of supercritical fluid chromatography separations coupled to mass spectrometry instrumentation for the exploration, profiling, and quantitation of metabolites during the last two decades. The selection of metabolites chosen for this article have direct applications in preclinical models of disease and clinical applications as potential biomarkers of disease including lipids, steroid hormones, bile acids, polar metabolites, peptides, and proteins.
AB - While supercritical fluid chromatography was developed over 50 years ago, it is only over the past 15 to 20 years that it has become routinely utilized. Along with the commercialization of a new generation of instruments, during the last 20 years supercritical fluid chromatography has improved performance, reliability, and robustness. Supercritical fluid chromatography is fully compatible with mass spectrometric techniques. This review compiles the application of supercritical fluid chromatography separations coupled to mass spectrometry instrumentation for the exploration, profiling, and quantitation of metabolites during the last two decades. The selection of metabolites chosen for this article have direct applications in preclinical models of disease and clinical applications as potential biomarkers of disease including lipids, steroid hormones, bile acids, polar metabolites, peptides, and proteins.
KW - biomarker discovery
KW - metabolomics
KW - supercritical fluids
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U2 - 10.1002/jssc.202000805
DO - 10.1002/jssc.202000805
M3 - Review article
C2 - 33085158
AN - SCOPUS:85096681763
SN - 1615-9306
VL - 44
SP - 448
EP - 463
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 1
ER -