TY - JOUR
T1 - Subcellular localization of luteinizing hormone releasing hormone degrading activity in the hypothalamus
AU - Richard Parker, C.
AU - Foreman, Mark M.
AU - Porter, John C.
PY - 1979/10/5
Y1 - 1979/10/5
N2 - The in vitro degradation of endogenous as well as exogenous luteinizing hormone releasing hormone (LHRH) by subcellular fractions of rat hypothalamic tissue was studied. Endogenous LHRH, localized in isolated nerve terminals (synaptosomes), was found to be resistant to enzymatic degradation (60 min, 37 °C) as long as the synaptosomal membrane remained intact. Endogenous LHRH was rapidly degraded by the 900 × g supernatant fluid and cytosol by not myelin/microsomes, intact synaptosomes, or mitochondria. Lysed synaptosomes rapidly degraded exogenous LHRH. The LHRH degrading activity of synaptosomes was highly concentrated in the 'synaptosomal', i.e., the cytosol of the nerve terminal. These data suggest that the LHRH degrading activity of the rat hypothalamus is a readily solubilized component of neurons, and possibly of non-neuronal cells.
AB - The in vitro degradation of endogenous as well as exogenous luteinizing hormone releasing hormone (LHRH) by subcellular fractions of rat hypothalamic tissue was studied. Endogenous LHRH, localized in isolated nerve terminals (synaptosomes), was found to be resistant to enzymatic degradation (60 min, 37 °C) as long as the synaptosomal membrane remained intact. Endogenous LHRH was rapidly degraded by the 900 × g supernatant fluid and cytosol by not myelin/microsomes, intact synaptosomes, or mitochondria. Lysed synaptosomes rapidly degraded exogenous LHRH. The LHRH degrading activity of synaptosomes was highly concentrated in the 'synaptosomal', i.e., the cytosol of the nerve terminal. These data suggest that the LHRH degrading activity of the rat hypothalamus is a readily solubilized component of neurons, and possibly of non-neuronal cells.
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U2 - 10.1016/0006-8993(79)90846-1
DO - 10.1016/0006-8993(79)90846-1
M3 - Article
C2 - 226218
AN - SCOPUS:0018392496
SN - 0006-8993
VL - 174
SP - 221
EP - 228
JO - Brain Research
JF - Brain Research
IS - 2
ER -