Structure of the yeast mitochondrial

Alexey Amunts; Alan Brown; Xiao Chen Bai; Jose L. Llácer; Tanweer Hussain; Paul Emsley; Fei Long; Garib Murshudov; Sjors H.W. Scheres; V. Ramakrishnan

doi:10.1126/science.1249410

Structure of the yeast mitochondrial

Alexey Amunts, Alan Brown, Xiao Chen Bai, Jose L. Llácer, Tanweer Hussain, Paul Emsley, Fei Long, Garib Murshudov, Sjors H.W. Scheres, V. Ramakrishnan

Research output: Contribution to journal › Article › peer-review

451 Scopus citations

Abstract

Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts.We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.

Original language	English (US)
Pages (from-to)	1485-1489
Number of pages	5
Journal	Science
Volume	343
Issue number	6178
DOIs	https://doi.org/10.1126/science.1249410
State	Published - 2014

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abstract = "Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts.We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.",

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AU - Amunts, Alexey

AU - Brown, Alan

AU - Bai, Xiao Chen

AU - Llácer, Jose L.

AU - Hussain, Tanweer

AU - Emsley, Paul

AU - Long, Fei

AU - Murshudov, Garib

AU - Scheres, Sjors H.W.

AU - Ramakrishnan, V.

PY - 2014

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AB - Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts.We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.

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Structure of the yeast mitochondrial

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