Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein

Matthew M. Skinner; Hong Zhang; Dale H. Leschnitzer; Yue Guan; Henry Bellamy; Robert M. Sweet; Carla W. Gray; Ruud N H Konings; Andrew H J Wang; Thomas C. Terwilliger

doi:10.1073/pnas.91.6.2071

Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein

Matthew M. Skinner, Hong Zhang, Dale H. Leschnitzer, Yue Guan, Henry Bellamy, Robert M. Sweet, Carla W. Gray, Ruud N H Konings, Andrew H J Wang, Thomas C. Terwilliger

Research output: Contribution to journal › Article › peer-review

116 Scopus citations

Abstract

The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47 → methionine mutant proteins with x-ray diffraction data phased to 2.5 Å resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 Å resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein- single-stranded DNA complex.

Original language	English (US)
Pages (from-to)	2071-2075
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	6
DOIs	https://doi.org/10.1073/pnas.91.6.2071
State	Published - Mar 15 1994

Keywords

DNA binding protein
protein structure
protein-DNA complex
synchrotron radiation
x-ray crystallography

ASJC Scopus subject areas

General

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10.1073/pnas.91.6.2071

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Skinner, M. M., Zhang, H., Leschnitzer, D. H., Guan, Y., Bellamy, H., Sweet, R. M., Gray, C. W., Konings, R. N. H., Wang, A. H. J., & Terwilliger, T. C. (1994). Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein. Proceedings of the National Academy of Sciences of the United States of America, 91(6), 2071-2075. https://doi.org/10.1073/pnas.91.6.2071

Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein. / Skinner, Matthew M.; Zhang, Hong; Leschnitzer, Dale H. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 6, 15.03.1994, p. 2071-2075.

Research output: Contribution to journal › Article › peer-review

Skinner, MM, Zhang, H, Leschnitzer, DH, Guan, Y, Bellamy, H, Sweet, RM, Gray, CW, Konings, RNH, Wang, AHJ & Terwilliger, TC 1994, 'Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 6, pp. 2071-2075. https://doi.org/10.1073/pnas.91.6.2071

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title = "Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein",

abstract = "The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47 → methionine mutant proteins with x-ray diffraction data phased to 2.5 {\AA} resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 {\AA} resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein- single-stranded DNA complex.",

keywords = "DNA binding protein, protein structure, protein-DNA complex, synchrotron radiation, x-ray crystallography",

author = "Skinner, {Matthew M.} and Hong Zhang and Leschnitzer, {Dale H.} and Yue Guan and Henry Bellamy and Sweet, {Robert M.} and Gray, {Carla W.} and Konings, {Ruud N H} and Wang, {Andrew H J} and Terwilliger, {Thomas C.}",

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T1 - Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein

AU - Skinner, Matthew M.

AU - Zhang, Hong

AU - Leschnitzer, Dale H.

AU - Guan, Yue

AU - Bellamy, Henry

AU - Sweet, Robert M.

AU - Gray, Carla W.

AU - Konings, Ruud N H

AU - Wang, Andrew H J

AU - Terwilliger, Thomas C.

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AB - The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47 → methionine mutant proteins with x-ray diffraction data phased to 2.5 Å resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 Å resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein- single-stranded DNA complex.

KW - DNA binding protein

KW - protein structure

KW - protein-DNA complex

KW - synchrotron radiation

KW - x-ray crystallography

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Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein

Abstract

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