Structural analysis of 1-aminocyclopropane-1-carboxylate deaminase: Observation of an aminyl intermediate and identification of Tyr294 as the active-site nucleophile

Subramanian Karthikeyan; Zongbao Zhao; Chailin Kao; Qingxian Zhou; Zhihua Tao; Hong Zhang; Hung Wen Liu

doi:10.1002/anie.200453353

Structural analysis of 1-aminocyclopropane-1-carboxylate deaminase: Observation of an aminyl intermediate and identification of Tyr294 as the active-site nucleophile

Subramanian Karthikeyan, Zongbao Zhao, Chailin Kao, Qingxian Zhou, Zhihua Tao, Hong Zhang, Hung Wen Liu

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Finding an angle of attack: Structural studies of 1-aminocyclopropane-1- carboxylate (ACC) deaminase complexed with the tight-binding inhibitor 1-amino-cyclopropanephosphonate (ACP, see picture) revealed the existence of an aminyl adduct intermediate. The crystal structure of the enzyme and mutation studies suggest that the ring cleavage of ACC is initiated by nucleophilic attack by Tyr294.

Original language	English (US)
Pages (from-to)	3425-3429
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	43
Issue number	26
DOIs	https://doi.org/10.1002/anie.200453353
State	Published - Jun 28 2004

Keywords

Enzyme catalysis
Enzymes
Proteins
Reaction mechanisms
Structure elucidation

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.200453353

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Structural analysis of 1-aminocyclopropane-1-carboxylate deaminase: Observation of an aminyl intermediate and identification of Tyr294 as the active-site nucleophile. / Karthikeyan, Subramanian; Zhao, Zongbao; Kao, Chailin et al.
In: Angewandte Chemie - International Edition, Vol. 43, No. 26, 28.06.2004, p. 3425-3429.

Research output: Contribution to journal › Article › peer-review

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abstract = "Finding an angle of attack: Structural studies of 1-aminocyclopropane-1- carboxylate (ACC) deaminase complexed with the tight-binding inhibitor 1-amino-cyclopropanephosphonate (ACP, see picture) revealed the existence of an aminyl adduct intermediate. The crystal structure of the enzyme and mutation studies suggest that the ring cleavage of ACC is initiated by nucleophilic attack by Tyr294.",

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AU - Kao, Chailin

AU - Zhou, Qingxian

AU - Tao, Zhihua

AU - Zhang, Hong

AU - Liu, Hung Wen

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KW - Enzymes

KW - Proteins

KW - Reaction mechanisms

KW - Structure elucidation

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Structural analysis of 1-aminocyclopropane-1-carboxylate deaminase: Observation of an aminyl intermediate and identification of Tyr294 as the active-site nucleophile

Abstract

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