Abstract
A stable isotope dilution assay for galactitol, in amniotic fluid has been developed using selected ion monitoring chemical ionization gas chromatography-mass spectrometry of the hexaacetate derivative. [1,1-2H2]Galactitol was synthesized for use as the internal standard. Galactitol is a component of normal amniotic fluid with a mean concentration of 0.70 ± 0.18 μmol/liter (n = 5). The amniotic fluid of a fetus with galactosemia had a concentration of 7.96 μmol/liter. Mannitol, sorbitol, and inositol were also found to be normal constituents of amniotic fluid. This stable isotope dilution assay is a rapid accurate method for measurement of galactitol in amniotic fluid for prenatal diagnosis of galactosemia.
Original language | English (US) |
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Pages (from-to) | 714-718 |
Number of pages | 5 |
Journal | Pediatric Research |
Volume | 18 |
Issue number | 8 |
DOIs | |
State | Published - Aug 1984 |
Externally published | Yes |
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health