TY - JOUR
T1 - Splenic B Cells Act as Antigen Presenting Cells for the Induction of Anterior Chamber-Associated Immune Deviation
AU - Skelsey, Molly E.
AU - Mayhew, Elizabeth
AU - Niederkorn, Jerry Y.
PY - 2003/12
Y1 - 2003/12
N2 - PURPOSE. To characterize the role of B cells in the induction of anterior chamber-associated immune deviation (ACAID). METHODS. An in vitro model of the ACAID spleen was used to recapitulate the events that occur when antigen is introduced into the anterior chamber of the eye and culminates in the appearance of antigen-specific, CD8+ suppressor cells. RESULTS. In vitro-generated suppressor cells mimicked those produced by anterior chamber injection of antigen, as shown by their antigen specificity, surface expression of CD8, and capacity to suppress DTH, which is mediated by previously immunized T cells. B cells were found to be necessary for suppressor cell development. The B cell receptor (BCR) was necessary for the induction of ACAID and conveyed antigen specificity to the suppressor T cells. Lysosomal acidification of internalized antigen was necessary for B cells to induce ACAID; however, transporter of antigen processing (TAP) was not required for the generation of ACAID. CONCLUSIONS. The results suggest that B cells use the BCR to capture and internalize antigen from ACAID-inducing macrophages. Lysosomal acidification of the captured antigen is essential for the processing of the ACAID antigen before TAP-independent presentation to suppressor cells.
AB - PURPOSE. To characterize the role of B cells in the induction of anterior chamber-associated immune deviation (ACAID). METHODS. An in vitro model of the ACAID spleen was used to recapitulate the events that occur when antigen is introduced into the anterior chamber of the eye and culminates in the appearance of antigen-specific, CD8+ suppressor cells. RESULTS. In vitro-generated suppressor cells mimicked those produced by anterior chamber injection of antigen, as shown by their antigen specificity, surface expression of CD8, and capacity to suppress DTH, which is mediated by previously immunized T cells. B cells were found to be necessary for suppressor cell development. The B cell receptor (BCR) was necessary for the induction of ACAID and conveyed antigen specificity to the suppressor T cells. Lysosomal acidification of internalized antigen was necessary for B cells to induce ACAID; however, transporter of antigen processing (TAP) was not required for the generation of ACAID. CONCLUSIONS. The results suggest that B cells use the BCR to capture and internalize antigen from ACAID-inducing macrophages. Lysosomal acidification of the captured antigen is essential for the processing of the ACAID antigen before TAP-independent presentation to suppressor cells.
UR - http://www.scopus.com/inward/record.url?scp=0344851544&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344851544&partnerID=8YFLogxK
U2 - 10.1167/iovs.03-0768
DO - 10.1167/iovs.03-0768
M3 - Article
C2 - 14638723
AN - SCOPUS:0344851544
SN - 0146-0404
VL - 44
SP - 5242
EP - 5251
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 12
ER -