PICK1 is a protein kinase C (PKC) α-binding protein initially identified using the yeast two-hybrid system. Here we report that PICK1 contains a PDZ domain that interacts specifically with a previously unidentified PDZ-binding domain (QSAV) at the extreme COOH terminus of PKCα and that mutation of a putative carboxylate-binding loop within the PICK1 PDZ domain abolishes this interaction. The PDZ-binding domain in PKCα is absent from other PKC isoforms that do not interact with PICK1. We also demonstrate that PICK1 can homooligomerize through sequences that are distinct from the carboxylate-binding loop, suggesting that self-association and PKCα binding are not mutually exclusive. A Caenorhabditis elegans PICK1-like protein is also able to bind to PKCα, suggesting a conservation of function through evolution. Association of PKCα with PICK1 provides a potential mechanism for the selective targeting of PKCα to unique subcellular sites.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Biological Chemistry|
|State||Published - Dec 19 1997|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology