TY - JOUR
T1 - Spatial organization of extracellular matrix and fibroblast activity
T2 - Effects of serum, transforming growth factor β, and fibronectin
AU - Fukamizu, Hidekazu
AU - Grinnell, Frederick
N1 - Funding Information:
We thank Pamala Pawelek and Drs. Richard Anderson suggestions. This research (GM31321) and the Kendall for help preparing photomicrographs and William Snell for their advice and was supported by grants from the NIH Health Care Products Co.
PY - 1990/10
Y1 - 1990/10
N2 - The goal of our research is to understand reciprocal relationships between cell function and tissue organization. We studied the regulation of fibroblast activity in an in vitro culture model that recapitulates in continuous fashion the cycle of events occurring during connective tissue repair. We present evidence that concomitant with spatial reorganization of the extracellular matrix, there was a dramatic decline in extracellular matrix synthesis and cell proliferation. Therefore, spatial reorganization was a crucial turning point for fibroblast activity. Factors that regulated the timing of spatial reorganization included serum, transforming growth factor β, and fibronectin. By accelerating spatial reorganization of the cultures, transforming growth factor β led to a relative decrease in cell proliferation and extracellular matrix synthesis. By retarding spatial reorganization of the cultures, fibronectin led to a relative increase in cell proliferation and extracellular matrix synthesis. The results indicate that spatial information in the three-dimensional cell-matrix interaction permits higher order, tissue-level regulation of fibroblast function.
AB - The goal of our research is to understand reciprocal relationships between cell function and tissue organization. We studied the regulation of fibroblast activity in an in vitro culture model that recapitulates in continuous fashion the cycle of events occurring during connective tissue repair. We present evidence that concomitant with spatial reorganization of the extracellular matrix, there was a dramatic decline in extracellular matrix synthesis and cell proliferation. Therefore, spatial reorganization was a crucial turning point for fibroblast activity. Factors that regulated the timing of spatial reorganization included serum, transforming growth factor β, and fibronectin. By accelerating spatial reorganization of the cultures, transforming growth factor β led to a relative decrease in cell proliferation and extracellular matrix synthesis. By retarding spatial reorganization of the cultures, fibronectin led to a relative increase in cell proliferation and extracellular matrix synthesis. The results indicate that spatial information in the three-dimensional cell-matrix interaction permits higher order, tissue-level regulation of fibroblast function.
UR - http://www.scopus.com/inward/record.url?scp=0025129750&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025129750&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(90)90197-I
DO - 10.1016/0014-4827(90)90197-I
M3 - Article
C2 - 2209728
AN - SCOPUS:0025129750
SN - 0014-4827
VL - 190
SP - 276
EP - 282
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -