SP100 expression modulates ETS1 transcriptional activity and inhibits cell invasion

John S. Yordy, Runzhao Li, Victor I. Sementchenko, Huiping Pei, Robin C. Muise-Helmericks, Dennis K. Watson

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


The ETS1 transcription factor is a member of the Ets family of conserved sequence-specific DNA-binding proteins. ETS1 has been shown to play important roles in various cellular processes such as proliferation, differentiation, lymplioid development, motility, invasion and angiogenesis. These diverse roles of ETS1 are likely to be dependent on specific protein interactions. To identify proteins that interact with ETS1, a yeast two-hybrid screen was conducted. Here, we describe the functional interaction between SP100 and ETS1. SP100 protein interacts with ETS1 both in vitro and in vivo. SP100 is localized to nuclear bodies and ETS1 expression alters the nuclear body morphology in living cells. SP100 negatively modulates ETS1 transcriptional activation of the MMP1 and uPA promoters in a dose-dependent manner, decreases the expression of these endogenous genes, and reduces ETS1 DNA binding. Expression of SP100 inhibits the invasion of breast cancer cells and is induced by Interferon-α, which has been shown to inhibit the invasion of cancer cells. These data demonstrate that SP100 modulates ETS1-dependent biological processes.

Original languageEnglish (US)
Pages (from-to)6654-6665
Number of pages12
Issue number39
StatePublished - Aug 26 2004


  • Ets
  • Invasion
  • MMP
  • SP100
  • Transcription
  • UPA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research


Dive into the research topics of 'SP100 expression modulates ETS1 transcriptional activity and inhibits cell invasion'. Together they form a unique fingerprint.

Cite this