TY - JOUR
T1 - Single Particle Automated Raman Trapping Analysis
AU - Penders, Jelle
AU - Pence, Isaac J.
AU - Horgan, Conor C.
AU - Bergholt, Mads S.
AU - Wood, Christopher S.
AU - Najer, Adrian
AU - Kauscher, Ulrike
AU - Nagelkerke, Anika
AU - Stevens, Molly M.
N1 - Funding Information:
J.P. and C.C.H. acknowledge funding from the NanoMed Marie Skłodowska-Curie ITN from the H2020 programme under grant number 676137. I.J.P. acknowledges support from the Whitaker International Program, Institute of International Education, United States of America. M.S.B. acknowledges support from H2020 through the Individual Marie Skłodowska-Curie Fellowship “IMAGINE” (701713). C.S.W. acknowledges support from the i-sense Engineering and Physical Sciences Research Council (EPSRC) IRC in Early Warning Sensing Systems for Infectious Diseases (EP/ K031953/1; www.i-sense. org.uk). A.Najer was supported by a Swiss National Science Foundation Early Postdoc Mobility Fellowship (P2BSP2_168751), which is gratefully acknowledged. U.K. acknowledges support from the Deutsche Forschungsgemeinschaft [KA 4370/1-1]. A. Nagelkerke acknowledges support from NIHR Imperial Biomedical Research Centre and the Institute of Cancer Research, London, through the joint Cancer Research Centre of Excellence (CRCE). M.M.S. acknowledges a Wellcome Trust Senior Investigator Award (098411/Z/12/Z).
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Enabling concurrent, high throughput analysis of single nanoparticles would greatly increase the capacity to study size, composition and inter and intra particle population variance with applications in a wide range of fields from polymer science to drug delivery. Here, we present a comprehensive platform for Single Particle Automated Raman Trapping Analysis (SPARTA) able to integrally analyse nanoparticles ranging from synthetic polymer particles to liposomes without any modification. With the developed highly controlled automated trapping process, single nanoparticles are analysed with high throughput and sensitivity to resolve particle mixtures, obtain detailed compositional spectra of complex particles, track sequential functionalisations, derive particle sizes and monitor the dynamics of click reactions occurring on the nanoparticle surface. The SPARTA platform opens up a wide range of new avenues for nanoparticle research through label-free integral high-throughput single particle analysis, overcoming key limitations in sensitivity and specificity of existing bulk analysis methods.
AB - Enabling concurrent, high throughput analysis of single nanoparticles would greatly increase the capacity to study size, composition and inter and intra particle population variance with applications in a wide range of fields from polymer science to drug delivery. Here, we present a comprehensive platform for Single Particle Automated Raman Trapping Analysis (SPARTA) able to integrally analyse nanoparticles ranging from synthetic polymer particles to liposomes without any modification. With the developed highly controlled automated trapping process, single nanoparticles are analysed with high throughput and sensitivity to resolve particle mixtures, obtain detailed compositional spectra of complex particles, track sequential functionalisations, derive particle sizes and monitor the dynamics of click reactions occurring on the nanoparticle surface. The SPARTA platform opens up a wide range of new avenues for nanoparticle research through label-free integral high-throughput single particle analysis, overcoming key limitations in sensitivity and specificity of existing bulk analysis methods.
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U2 - 10.1038/s41467-018-06397-6
DO - 10.1038/s41467-018-06397-6
M3 - Article
C2 - 30323298
AN - SCOPUS:85054894160
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 4256
ER -