Single-molecule fluorescence to study molecularmotors

Hyokeun Park, Erdal Toprak, Paul R. Selvin

Research output: Contribution to journalReview articlepeer-review

71 Scopus citations


Molecular motors, which use energy from ATP hydrolysis to take nanometer-scale steps with run-lengths on the order of micrometers, have important roles in areas such as transport and mitosis in living organisms. New techniques have recently been developed to measure these small movements at the single-molecule level. In particular, fluorescence imaging has contributed to the accurate measurement of this tiny movement. We introduce three single-molecule fluorescence imaging techniques which can find the position of a fluorophore with accuracy in the range of a few nanometers. These techniques are named after Hollywood animation characters : Fluorescence Imaging with One Nanometer Accuracy (FIONA), Single-molecule High-REsolution Colocalization (SHREC), and Defocused Orientation and Position Imaging (DOPI). We explain new understanding of molecular motors obtained from measurements using these techniques.

Original languageEnglish (US)
Pages (from-to)87-111
Number of pages25
JournalQuarterly Reviews of Biophysics
Issue number1
StatePublished - Feb 2007

ASJC Scopus subject areas

  • Biophysics


Dive into the research topics of 'Single-molecule fluorescence to study molecularmotors'. Together they form a unique fingerprint.

Cite this