Silencing of filamin A gene expression inhibits Ca2+-sensing receptor signaling

Chunfa Huang, Zhenzhen Wu, Kristine M. Hujer, R. Tyler Miller

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+-sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.

Original languageEnglish (US)
Pages (from-to)1795-1800
Number of pages6
JournalFEBS Letters
Issue number7
StatePublished - Mar 20 2006


  • Ca-sensing receptor
  • Filamin A
  • MAP kinase
  • Signaling
  • siRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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