TY - JOUR
T1 - Sex-dependent attenuating effects of capsaicin administration on the mechanoreflex in healthy rats
AU - Butenas, Alec L.E.
AU - Ishizawa, Rie
AU - Rollins, Korynne S.
AU - Mizuno, Masaki
AU - Copp, Steven W.
N1 - Publisher Copyright:
Copyright © 2023 the American Physiological Society.
PY - 2023/8
Y1 - 2023/8
N2 - Stimulation of mechanically sensitive channels on the sensory endings of group III and IV thin fiber muscle afferents activates the mechanoreflex, which contributes to reflex increases in sympathetic nerve activity (SNA) and blood pressure during exercise. Accumulating evidence suggests that activation of the nonselective cation channel transient receptor potential vanilloid-1 (TRPV1) on the sensory endings of thin fiber afferents with capsaicin may attenuate mechanosensation. However, no study has investigated the effect of capsaicin on the mechanoreflex. We tested the hypothesis that in male and female decerebrate, unanesthetized rats, the injection of capsaicin (0.05 lg) into the arterial supply of the hindlimb reduces the pressor and renal SNA (RSNA) response to 30 s of 1 Hz rhythmic hindlimb muscle stretch (a model of isolated mechanoreflex activation). In male rats (n ¼ 8), capsaicin injection significantly reduced the integrated blood pressure (blood pressure index or BPI: pre, 363 ± 78; post, 211 ± 88 mmHg·s; P ¼ 0.023) and RSNA [$DRSNA; pre, 687 ± 206; post, 216 ± 80 arbitrary units (au), P ¼ 0.049] response to hindlimb muscle stretch. In female rats (n ¼ 8), capsaicin injection had no significant effect on the pressor (BPI; pre: 277 ± 67; post: 207 ± 77 mmHg·s; P ¼ 0.343) or RSNA ($DRSNA: pre, 697 ± 123; post, 440 ± 183 au; P ¼ 0.307) response to hindlimb muscle stretch. The data suggest that the injection of capsaicin into the hindlimb arterial supply to stimulate TRPV1 on the sensory endings of thin fiber muscle afferents attenuates the mechanoreflex in healthy male, but not female, rats. The findings may carry important implications for chronic conditions in which an exaggerated mechanoreflex contributes to aberrant sympathoexcitation during exercise.
AB - Stimulation of mechanically sensitive channels on the sensory endings of group III and IV thin fiber muscle afferents activates the mechanoreflex, which contributes to reflex increases in sympathetic nerve activity (SNA) and blood pressure during exercise. Accumulating evidence suggests that activation of the nonselective cation channel transient receptor potential vanilloid-1 (TRPV1) on the sensory endings of thin fiber afferents with capsaicin may attenuate mechanosensation. However, no study has investigated the effect of capsaicin on the mechanoreflex. We tested the hypothesis that in male and female decerebrate, unanesthetized rats, the injection of capsaicin (0.05 lg) into the arterial supply of the hindlimb reduces the pressor and renal SNA (RSNA) response to 30 s of 1 Hz rhythmic hindlimb muscle stretch (a model of isolated mechanoreflex activation). In male rats (n ¼ 8), capsaicin injection significantly reduced the integrated blood pressure (blood pressure index or BPI: pre, 363 ± 78; post, 211 ± 88 mmHg·s; P ¼ 0.023) and RSNA [$DRSNA; pre, 687 ± 206; post, 216 ± 80 arbitrary units (au), P ¼ 0.049] response to hindlimb muscle stretch. In female rats (n ¼ 8), capsaicin injection had no significant effect on the pressor (BPI; pre: 277 ± 67; post: 207 ± 77 mmHg·s; P ¼ 0.343) or RSNA ($DRSNA: pre, 697 ± 123; post, 440 ± 183 au; P ¼ 0.307) response to hindlimb muscle stretch. The data suggest that the injection of capsaicin into the hindlimb arterial supply to stimulate TRPV1 on the sensory endings of thin fiber muscle afferents attenuates the mechanoreflex in healthy male, but not female, rats. The findings may carry important implications for chronic conditions in which an exaggerated mechanoreflex contributes to aberrant sympathoexcitation during exercise.
KW - TRPV1 channels
KW - blood pressure
KW - exercise pressor reflex
KW - sensory neurons
UR - http://www.scopus.com/inward/record.url?scp=85165521536&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85165521536&partnerID=8YFLogxK
U2 - 10.1152/ajpheart.00237.2023
DO - 10.1152/ajpheart.00237.2023
M3 - Article
C2 - 37389947
AN - SCOPUS:85165521536
SN - 0193-1849
VL - 325
SP - H372-H384
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
IS - 2
ER -