Sephadex G-10 adsorption chromatography of purines and related compounds

Lawrence Sweetman; William L. Nyhan

doi:10.1016/s0021-9673(01)80545-8

Sephadex G-10 adsorption chromatography of purines and related compounds

Lawrence Sweetman, William L. Nyhan

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The adsorption chromatography of purines and related heterocyclic compounds on Sephadex G-10 was investigated under different conditions of pH, ionic strength and flow rate and found to give excellent separations. The elution volumes were highly reproducible and recovery quantitative. A relation between a log function of the elution volumes and substituents on the purine ring was shown and the additivity of the resulting Δ log V₆° values used to predict elution volumes. Some analytical and preparative applications have been undertaken.

Original language	English (US)
Pages (from-to)	662-675
Number of pages	14
Journal	Journal of Chromatography A
Volume	32
Issue number	C
DOIs	https://doi.org/10.1016/s0021-9673(01)80545-8
State	Published - 1968
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/s0021-9673(01)80545-8

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abstract = "The adsorption chromatography of purines and related heterocyclic compounds on Sephadex G-10 was investigated under different conditions of pH, ionic strength and flow rate and found to give excellent separations. The elution volumes were highly reproducible and recovery quantitative. A relation between a log function of the elution volumes and substituents on the purine ring was shown and the additivity of the resulting Δ log V6° values used to predict elution volumes. Some analytical and preparative applications have been undertaken.",

author = "Lawrence Sweetman and Nyhan, {William L.}",

note = "Funding Information: * This investigation was supported by Public Health Service Research Grant No. I-ID o2Gog ancl Training Grant No, Tl I-ID IG from the National Institute of Child Health and Human Development.",

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T1 - Sephadex G-10 adsorption chromatography of purines and related compounds

AU - Sweetman, Lawrence

AU - Nyhan, William L.

N1 - Funding Information: * This investigation was supported by Public Health Service Research Grant No. I-ID o2Gog ancl Training Grant No, Tl I-ID IG from the National Institute of Child Health and Human Development.

PY - 1968

Y1 - 1968

N2 - The adsorption chromatography of purines and related heterocyclic compounds on Sephadex G-10 was investigated under different conditions of pH, ionic strength and flow rate and found to give excellent separations. The elution volumes were highly reproducible and recovery quantitative. A relation between a log function of the elution volumes and substituents on the purine ring was shown and the additivity of the resulting Δ log V6° values used to predict elution volumes. Some analytical and preparative applications have been undertaken.

AB - The adsorption chromatography of purines and related heterocyclic compounds on Sephadex G-10 was investigated under different conditions of pH, ionic strength and flow rate and found to give excellent separations. The elution volumes were highly reproducible and recovery quantitative. A relation between a log function of the elution volumes and substituents on the purine ring was shown and the additivity of the resulting Δ log V6° values used to predict elution volumes. Some analytical and preparative applications have been undertaken.

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JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - C

ER -

Sephadex G-10 adsorption chromatography of purines and related compounds

Abstract

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