TY - JOUR
T1 - Seipin and Nem1 establish discrete ER subdomains to initiate yeast lipid droplet biogenesis
AU - Choudhary, Vineet
AU - El Atab, Ola
AU - Mizzon, Giulia
AU - Prinz, William A.
AU - Schneiter, Roger
N1 - Funding Information:
This work was supported by the Swiss National Science Foundation (grant 31003A_17303), the Novartis Foundation for medical-biological research (grant 19B140), and the Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases. V. Choudhary was supported by European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement no. 747536. The authors declare no competing financial interests.
Funding Information:
We thank Dr. Paolo Ronchi and the Electron Microscopy Core Facility of the European Molecular Biology Laboratory, Heidelberg, Germany, for expert technical assistance in CLEM imaging. This work was supported by the Swiss National Science Foundation (grant 31003A_17303), the Novartis Foundation for medical-biological research (grant 19B140), and the Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases. V. Choudhary was supported by European Union's Horizon 2020 Research and Innovation Programme under the Marie Sk?odowska-Curie grant agreement no. 747536.
Publisher Copyright:
© 2020 Choudhary et al. This article is distributed under the terms of an Attribution-Noncommercial-Share Alike-No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
PY - 2020/7/6
Y1 - 2020/7/6
N2 - Lipid droplets (LDs) are fat storage organelles that originate from the endoplasmic reticulum (ER). Relatively little is known about how sites of LD formation are selected and which proteins/lipids are necessary for the process. Here, we show that LDs induced by the yeast triacylglycerol (TAG)-synthases Lro1 and Dga1 are formed at discrete ER subdomains defined by seipin (Fld1), and a regulator of diacylglycerol (DAG) production, Nem1. Fld1 and Nem1 colocalize to ER-LD contact sites. We find that Fld1 and Nem1 localize to ER subdomains independently of each other and of LDs, but both are required for the subdomains to recruit the TAG-synthases and additional LD biogenesis factors: Yft2, Pex30, Pet10, and Erg6. These subdomains become enriched in DAG. We conclude that Fld1 and Nem1 are both necessary to recruit proteins to ER subdomains where LD biogenesis occurs.
AB - Lipid droplets (LDs) are fat storage organelles that originate from the endoplasmic reticulum (ER). Relatively little is known about how sites of LD formation are selected and which proteins/lipids are necessary for the process. Here, we show that LDs induced by the yeast triacylglycerol (TAG)-synthases Lro1 and Dga1 are formed at discrete ER subdomains defined by seipin (Fld1), and a regulator of diacylglycerol (DAG) production, Nem1. Fld1 and Nem1 colocalize to ER-LD contact sites. We find that Fld1 and Nem1 localize to ER subdomains independently of each other and of LDs, but both are required for the subdomains to recruit the TAG-synthases and additional LD biogenesis factors: Yft2, Pex30, Pet10, and Erg6. These subdomains become enriched in DAG. We conclude that Fld1 and Nem1 are both necessary to recruit proteins to ER subdomains where LD biogenesis occurs.
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U2 - 10.1083/JCB.201910177
DO - 10.1083/JCB.201910177
M3 - Article
C2 - 32349126
AN - SCOPUS:85084170404
SN - 0021-9525
VL - 219
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 7
M1 - 201910177
ER -